This Program Project represents a coordinated and mutually supporting effort to pursue research in structural biology where electron diffraction and electron microscopy play a key role and to advance the technology that enables this research. Three projects within the Program represent applications of the methods of electron crystallography and microscopy, as well as of the instrumentation supported by the Program, to specific problems in structural biology. These applications focus on aspects of the eukaryotic cytoskeleton and include: (Project A) Studies of the structure of tubulin and its interactions with ligands and other proteins that affect the function of the microtubule cytoskeleton;(Project B) Studies of the apparatus in the inner ear that converts sound to nerve signals and controls sensitivity of hearing;(Project C) Studies of the proteins that connect microtubules to chromosomes and ensure proper chromosome segregation during cell division. Additional, separately funded research projects which rely to a smaller extent on equipment and facilities supported by the Program Project are also included as Associate member projects. The Administrative Core (Project D) maintains the major equipment, supports computer resources, continues our efforts to improve the quality and throughput of electron microscopy data, and provides a sense of intellectual community and common identity for all the participants in the Program Project.

Public Health Relevance

Our structural studies of several medically important protein complexes that play essential roles within the cell provide the basis for understanding their normal function as well as failures in these functions that are associated with disease and birth defects.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM051487-17
Application #
8299582
Study Section
Special Emphasis Panel (ZRG1-CB-B (40))
Program Officer
Flicker, Paula F
Project Start
1997-07-01
Project End
2015-05-31
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
17
Fiscal Year
2012
Total Cost
$1,815,996
Indirect Cost
$778,340
Name
Lawrence Berkeley National Laboratory
Department
Biochemistry
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
Han, Bong-Gyoon; Watson, Zoe; Kang, Hannah et al. (2016) Long shelf-life streptavidin support-films suitable for electron microscopy of biological macromolecules. J Struct Biol 195:238-44
Hurley, James H; Nogales, Eva (2016) Next-generation electron microscopy in autophagy research. Curr Opin Struct Biol 41:211-216
Borisy, Gary; Heald, Rebecca; Howard, Jonathon et al. (2016) Microtubules: 50 years on from the discovery of tubulin. Nat Rev Mol Cell Biol 17:322-8
Kellogg, Elizabeth H; Howes, Stuart; Ti, Shih-Chieh et al. (2016) Near-atomic cryo-EM structure of PRC1 bound to the microtubule. Proc Natl Acad Sci U S A 113:9430-9
Sun, Jing; Jiang, Xi; Lund, Reidar et al. (2016) Self-assembly of crystalline nanotubes from monodisperse amphiphilic diblock copolypeptoid tiles. Proc Natl Acad Sci U S A 113:3954-9
Berleman, James E; Zemla, Marcin; Remis, Jonathan P et al. (2016) Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior. ISME J 10:2620-2632
Young, Lindsey N; Cho, Kelvin; Lawrence, Rosalie et al. (2016) Dynamics and architecture of the NRBF2-containing phosphatidylinositol 3-kinase complex I of autophagy. Proc Natl Acad Sci U S A 113:8224-9
Shamir, Eliah R; Coutinho, Kester; Georgess, Dan et al. (2016) Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination. Biol Open 5:1216-28
Jiang, Fuguo; Taylor, David W; Chen, Janice S et al. (2016) Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Science 351:867-71
Sun, Jing; Jiang, Xi; Siegmund, Aaron et al. (2016) Morphology and Proton Transport in Humidified Phosphonated Peptoid Block Copolymers. Macromolecules 49:3083-3090

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