This Program Project represents a coordinated and mutually supporting effort to pursue research in structural biology where electron diffraction and electron microscopy play a key role and to advance the technology that enables this research. Three projects within the Program represent applications of the methods of electron crystallography and microscopy, as well as of the instrumentation supported by the Program, to specific problems in structural biology. These applications focus on aspects of the eukaryotic cytoskeleton and include: (Project A) Studies of the structure of tubulin and its interactions with ligands and other proteins that affect the function of the microtubule cytoskeleton;(Project B) Studies of the apparatus in the inner ear that converts sound to nerve signals and controls sensitivity of hearing;(Project C) Studies of the proteins that connect microtubules to chromosomes and ensure proper chromosome segregation during cell division. Additional, separately funded research projects which rely to a smaller extent on equipment and facilities supported by the Program Project are also included as Associate member projects. The Administrative Core (Project D) maintains the major equipment, supports computer resources, continues our efforts to improve the quality and throughput of electron microscopy data, and provides a sense of intellectual community and common identity for all the participants in the Program Project.
Our structural studies of several medically important protein complexes that play essential roles within the cell provide the basis for understanding their normal function as well as failures in these functions that are associated with disease and birth defects.
|Holmes, Dawn E; Giloteaux, Ludovic; Chaurasia, Akhilesh K et al. (2015) Evidence of Geobacter-associated phage in a uranium-contaminated aquifer. ISME J 9:333-46|
|Tsai, Wen-Ting; Hassan, Ahmed; Sarkar, Purbasha et al. (2014) From voxels to knowledge: a practical guide to the segmentation of complex electron microscopy 3D-data. J Vis Exp :e51673|
|Nakamura, Muneaki; Chen, Lu; Howes, Stuart C et al. (2014) Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol 9:693-7|
|Antonellis, Patrick J; Pollock, Lana M; Chou, Shih-Wei et al. (2014) ACF7 is a hair-bundle antecedent, positioned to integrate cuticular plate actin and somatic tubulin. J Neurosci 34:305-12|
|Beers, Keith M; Yakovlev, Sergey; Jackson, Andrew et al. (2014) Absence of Schroeder's paradox in a nanostructured block copolymer electrolyte membrane. J Phys Chem B 118:6785-91|
|Chen, X Chelsea; Wong, David T; Yakovlev, Sergey et al. (2014) Effect of morphology of nanoscale hydrated channels on proton conductivity in block copolymer electrolyte membranes. Nano Lett 14:4058-64|
|Shamir, Eliah R; Pappalardo, Elisa; Jorgens, Danielle M et al. (2014) Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. J Cell Biol 204:839-56|
|Howes, Stuart C; Alushin, Gregory M; Shida, Toshinobu et al. (2014) Effects of tubulin acetylation and tubulin acetyltransferase binding on microtubule structure. Mol Biol Cell 25:257-66|
|Remis, Jonathan P; Wei, Dongguang; Gorur, Amita et al. (2014) Bacterial social networks: structure and composition of Myxococcus xanthus outer membrane vesicle chains. Environ Microbiol 16:598-610|
|Alushin, Gregory M; Lander, Gabriel C; Kellogg, Elizabeth H et al. (2014) High-resolution microtubule structures reveal the structural transitions in ??-tubulin upon GTP hydrolysis. Cell 157:1117-29|
Showing the most recent 10 out of 75 publications