The long-term goals of the Program Project include the development of a detailed mechanistic model for actin filament branching mediated by the Arp2/3 complex and to characterize the role of the Arp2/3 complex in directed cell motility. Here, we aim at further characterizing the structures of key intermediates and their interactions along the activation pathway of the Arp2/3 complex (Aim 1).
For Aim 2, we will generate high resolution, three-dimensional (3D) structures of actin networks mediated by the complex in its cellular environment. This should allow us to determine how structural details of the Arp2/3-mediated actin networks are being modified by changes in cellular dynamics. We will apply high-resolution electron cryo-microscopy (cryo-EM), electron cryotomography and image analysis to achieve these aims.
|Volkmann, Niels (2014) The joys and perils of flexible fitting. Adv Exp Med Biol 805:137-55|
|Volkmann, Niels; Page, Christopher; Li, Rong et al. (2014) Three-dimensional reconstructions of actin filaments capped by Arp2/3 complex. Eur J Cell Biol 93:179-83|
|Xu, Xiao-Ping; Slaughter, Brian D; Volkmann, Niels (2013) Probabilistic determination of probe locations from distance data. J Struct Biol 184:75-82|
|Xu, X-P; Zhai, D; Kim, E et al. (2013) Three-dimensional structure of Bax-mediated pores in membrane bilayers. Cell Death Dis 4:e683|
|Yi, Kexi; Rubinstein, Boris; Li, Rong (2013) Symmetry breaking and polarity establishment during mouse oocyte maturation. Philos Trans R Soc Lond B Biol Sci 368:20130002|
|Li, Rong; Albertini, David F (2013) The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte. Nat Rev Mol Cell Biol 14:141-52|
|Pollard, Thomas D; De La Cruz, Enrique M (2013) Take advantage of time in your experiments: a guide to simple, informative kinetics assays. Mol Biol Cell 24:1103-10|
|Yi, Kexi; Rubinstein, Boris; Unruh, Jay R et al. (2013) Sequential actin-based pushing forces drive meiosis I chromosome migration and symmetry breaking in oocytes. J Cell Biol 200:567-76|
|Volkmann, Niels (2012) Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions. Curr Opin Cell Biol 24:141-7|
|Suraneni, Praveen; Rubinstein, Boris; Unruh, Jay R et al. (2012) The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration. J Cell Biol 197:239-51|
Showing the most recent 10 out of 43 publications