All of the sub-projects rely on the availability of high quality and high purity human cardiomyocytes that have been derived from patient-specific induced pluripotent stem cells (iPSCs).The overall objective of the Stem Cell Core is to provide the infrastructure, materials, and expertise to support the maintenance, derivation and differentiation of human pluripotent stem cells for PPG investigators. Although primary human cardiomyocytes could ostensibly be collected from patients, the logistics involved in harvesting cells from both control and type 2 diabetic populations make this approach infeasible. In addition, primary cardiac cells have a limited lifespan and cannot be expanded to provide the cell numbers necessary to meet the goals of the PPG. In contrast, human iPSCs can act as an indefatigable reservoir of human cardiomyocytes that can be cultured for over one year.
The specific aims of the Stem Cell Core are therefore to: (1) Reprogram new iPSC lines from non-diabetic and type 2 diabetic patients; (2) Generate reagents under strict quality control that are necessary for iPSC culture; (3) Provide training in the culture, handling, and differentiation of human iPSCs as required by PPG investigators; and (4) Supply purified iPSC-derived cardiomyocytes expressing enhanced green fluorescent protein to PPG investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM066730-14
Application #
9435135
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2018-03-01
Budget End
2019-02-28
Support Year
14
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
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