The defining characteristic of systems biology is that it seeks to develop a systems view of biology as opposed to a components view. This project aims to develop?in a feedback cycle that guides further experimentation?predictive computational models that capture the overall dynamics of the molecular network that underlies the shift of Neurospora from mycelia growth to asexual spore development through conidiation. Initial efforts will be directed towards construction of a predictive model for regulatory networks and correlation of this network with existing and new transcriptome and genomic data. This model will be tested and refined through incorporation of new data arising from Project 3 and, most importantly, via perturbation tests facilitated through the use of strains developed in Project 1 and characterized using the tools employed in Project 3. In addition, we will develop a comprehensive model of steady state metabolism in Neurospora and, by combining this model with our regulatory network model, make testable predictions about metabolic state corresponding to different gene expression states

Public Health Relevance

Filamentous fungi, typically known as molds, are common animal and plant pathogens, but they are also widely used as industrial strains to provide antibiotics, chemicals, enzymes, and Pharmaceuticals. We'd be dead without them but they can kill us. We seek to understand how genes and proteins work together to regulate fungal growth and development, so as to enhance the good things and control the bad things produced by fungi.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Program Projects (P01)
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Special Emphasis Panel (ZRG1-GGG-M)
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Dartmouth College
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Heller, Jens; Zhao, Jiuhai; Rosenfield, Gabriel et al. (2016) Characterization of Greenbeard Genes Involved in Long-Distance Kind Discrimination in a Microbial Eukaryote. PLoS Biol 14:e1002431
Heine, Daniela; Petereit, Linda; Schumann, Marcel R et al. (2016) The tetraspanin TSP3 of Neurospora crassa is a vacuolar membrane protein and shares characteristics with IDI proteins. Mycologia 108:581-9
Weichert, Martin; Lichius, Alexander; Priegnitz, Bert-Ewald et al. (2016) Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell-cell recognition and fusion. Proc Natl Acad Sci U S A 113:11877-11882
Mouriño-Pérez, Rosa Reyna; Riquelme, Meritxell; Callejas-Negrete, Olga Alicia et al. (2016) Microtubules and associated molecular motors in Neurospora crassa. Mycologia 108:515-27
Jonkers, Wilfried; Fischer, Monika S; Do, Hung P et al. (2016) Chemotropism and Cell Fusion in Neurospora crassa Relies on the Formation of Distinct Protein Complexes by HAM-5 and a Novel Protein HAM-14. Genetics 203:319-34
Doamekpor, Selom K; Lee, Joong-Won; Hepowit, Nathaniel L et al. (2016) Structure and function of the yeast listerin (Ltn1) conserved N-terminal domain in binding to stalled 60S ribosomal subunits. Proc Natl Acad Sci U S A 113:E4151-60
Pieuchot, Laurent; Lai, Julian; Loh, Rachel Ann et al. (2015) Cellular Subcompartments through Cytoplasmic Streaming. Dev Cell 34:410-20
Hurley, Jennifer; Loros, Jennifer J; Dunlap, Jay C (2015) Dissecting the mechanisms of the clock in Neurospora. Methods Enzymol 551:29-52
Fuller, Kevin K; Loros, Jennifer J; Dunlap, Jay C (2015) Fungal photobiology: visible light as a signal for stress, space and time. Curr Genet 61:275-88
Cabrera, Ilva E; Pacentine, Itallia V; Lim, Andrew et al. (2015) Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa. G3 (Bethesda) 5:2729-43

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