Abstract

Projects 1 and 2 will require high-end optical microscopy with single molecule tracking and single vesicle fusion and FRET capabilities to achieve aims 1 and 2, and 1 and 3, respectively. Project 3 will measure interbilayer distances by FLIC microscopy (aim 1) and determine synaptotagmin-induced changes in lipid order by ATR-FTIR spectroscopy (aim 3). Core A will provide the necessary state-of-the-art instrumentation, technical know-how, and personnel training to realize these aims of all three Projects. In addition, the Core will train users, provide customable software tools, and assist with image and data analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM072694-06A1
Application #
8119233
Study Section
Special Emphasis Panel (ZRG1-BCMB-S (40))
Project Start
Project End
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
6
Fiscal Year
2011
Total Cost
$100,500
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Nyenhuis, Sarah B; Cafiso, David S (2018) Choice of reconstitution protocol modulates the aggregation state of full-length membrane-reconstituted synaptotagmin-1. Protein Sci 27:1008-1012
Yavuz, Halenur; Kattan, Iman; Hernandez, Javier M et al. (2018) Arrest of trans-SNARE zippering uncovers loosely and tightly docked intermediates in membrane fusion. J Biol Chem 293:8645-8655
Liang, Binyong; Tamm, Lukas K (2018) Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics. Prog Nucl Magn Reson Spectrosc 105:41-53
Hussain, Syed Saad; Harris, Megan T; Kreutzberger, Alex J B et al. (2018) Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP. Mol Biol Cell 29:1238-1257
Blackburn, Matthew R; Hubbard, Caitlin; Kiessling, Volker et al. (2018) Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life. Glycobiology 28:108-121
Witkowska, Agata; Jablonski, Lukasz; Jahn, Reinhard (2018) A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation. Sci Rep 8:9422
Kiessling, Volker; Kreutzberger, Alex J B; Liang, Binyong et al. (2018) A molecular mechanism for calcium-mediated synaptotagmin-triggered exocytosis. Nat Struct Mol Biol 25:911-917
Kreutzberger, Alex J B; Kiessling, Volker; Liang, Binyong et al. (2017) Asymmetric Phosphatidylethanolamine Distribution Controls Fusion Pore Lifetime and Probability. Biophys J 113:1912-1915
Tamm, Lukas K (2017) Special Issue on Liposomes, Exosomes, and Virosomes. Biophys J 113:E1
Jakhanwal, Shrutee; Lee, Chung-Tien; Urlaub, Henning et al. (2017) An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly. EMBO J 36:1788-1802

Showing the most recent 10 out of 76 publications