The Analytical Resource Core will provide the researchers affiliated with the Program Project access to high performance analytical instrumentation, mass spectrometry (MS), and nuclear magnetic resonance spectroscopy (NMR), through training, experimental design and routine sample analysis. Coupled to these Instruments are nanocapillary chromatographs that allow separation and analysis of microscale experiments and culture extracts. The ability to analyze small amounts of material is crucial in the effort to employ MS and NMR In medium- and high-throughput screening campaigns for novel phosphonates. The Analytical Resource Core will also develop new methods and analyses that will accelerate the discovery and reengineering portions of the Research Projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM077596-08
Application #
8634115
Study Section
Special Emphasis Panel (ZRG1-BCMB-U)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
8
Fiscal Year
2014
Total Cost
$210,019
Indirect Cost
$77,515
Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
Shao, Zengyi; Zhao, Huimin (2014) Manipulating natural product biosynthetic pathways via DNA assembler. Curr Protoc Chem Biol 6:65-100
Cobb, Ryan E; Ning, Jonathan C; Zhao, Huimin (2014) DNA assembly techniques for next-generation combinatorial biosynthesis of natural products. J Ind Microbiol Biotechnol 41:469-77
Luo, Yunzi; Cobb, Ryan E; Zhao, Huimin (2014) Recent advances in natural product discovery. Curr Opin Biotechnol 30:230-7
Doroghazi, James R; Albright, Jessica C; Goering, Anthony W et al. (2014) A roadmap for natural product discovery based on large-scale genomics and metabolomics. Nat Chem Biol 10:963-8
Yu, Xiaomin; Price, Neil P J; Evans, Bradley S et al. (2014) Purification and characterization of phosphonoglycans from Glycomyces sp. strain NRRL B-16210 and Stackebrandtia nassauensis NRRL B-16338. J Bacteriol 196:1768-79
Wang, Meng; Beissner, Mirko; Zhao, Huimin (2014) Aryl-aldehyde formation in fungal polyketides: discovery and characterization of a distinct biosynthetic mechanism. Chem Biol 21:257-63
Agarwal, Vinayak; Peck, Spencer C; Chen, Jui-Hui et al. (2014) Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation. Chem Biol 21:125-35
Ju, Kou-San; Doroghazi, James R; Metcalf, William W (2014) Genomics-enabled discovery of phosphonate natural products and their biosynthetic pathways. J Ind Microbiol Biotechnol 41:345-56
Eriksen, Dawn T; Lian, Jiazhang; Zhao, Huimin (2014) Protein design for pathway engineering. J Struct Biol 185:234-42
Cioni, Joel P; Doroghazi, James R; Ju, Kou-San et al. (2014) Cyanohydrin phosphonate natural product from Streptomyces regensis. J Nat Prod 77:243-9

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