Core A provides expertise and resources that are essential for successful completion of each of the Projects within this Program. Core A is housed in the Ellison Stem Cell Core (ESCC), a purpose-designed and built facility providing access to tissue culture hoods, a number of Zeiss microscopes, live cell imaging capability, and hypoxia-ready incubators. Furthermore, Core A provides access to validated reagents for the culture and maintenance of human pluripotent stem cells, minimizing experimental variability that can occur between different vendors or batches. Core A also teaches and maintains a "best practices" environment that minimizes deviations that can lead to contamination and culture drift. However, perhaps of greatest importance are the advantages that result from the co-localization of POI investigators within the ESCC. By aggregating researchers from different disciplines, the ESCC aggregates expertise. This allows researchers from one laboratory to immediately benefit from advances made by other laboratories, facilitates the identification of important biological differences between pluripotent lines, and fosters collaborative efforts that benefit from contributions from multiple groups;a recent example being the adoption of vector-free methods for induced pluripotent stem cell (iPSC) generation. Core A will facilitate the goals of the overall POI through the provision of core services, by placing its knowledge and resources at the disposal of POI investigators, and by developing new knowledge and capabilities. During the current funding period Core A has made important contributions toward furthering our knowledge of stem cell biology. The current application proposes to continue in this vein by characterizing a new generation of "naive-like" human pluripotent stem cell lines, by developing culture conditions that will enhance the predictability with which human pluripotent stem cell lines generate tissues of interest, and by seeking to identify metabolic drivers of pluripotency and directed differentiation.
The ESCC will support the public health aspect of the individual projects and within core pursuits will advance our understanding of hESC toward the goal of control of expansion of these cells for clinical application.
|Ellen Kreipke, Rebecca; Wang, Yuliang; Miklas, Jason Wayne et al. (2016) Metabolic remodeling in early development and cardiomyocyte maturation. Semin Cell Dev Biol 52:84-92|
|Hofsteen, Peter; Robitaille, Aaron M; Chapman, Daniel Patrick et al. (2016) Quantitative proteomics identify DAB2 as a cardiac developmental regulator that inhibits WNT/Î²-catenin signaling. Proc Natl Acad Sci U S A 113:1002-7|
|Ruan, Jia-Ling; Tulloch, Nathaniel L; Razumova, Maria V et al. (2016) Mechanical Stress Conditioning and Electrical Stimulation Promote Contractility and Force Maturation of Induced Pluripotent Stem Cell-Derived Human Cardiac Tissue. Circulation 134:1557-1567|
|Pioner, JosÃ¨ Manuel; Racca, Alice W; Klaiman, Jordan M et al. (2016) Isolation and Mechanical Measurements of Myofibrils from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cell Reports 6:885-96|
|Xu, Zhuojin; Robitaille, Aaron M; Berndt, Jason D et al. (2016) Wnt/Î²-catenin signaling promotes self-renewal and inhibits the primed state transition in naÃ¯ve human embryonic stem cells. Proc Natl Acad Sci U S A 113:E6382-E6390|
|Roberts, Meredith A; Tran, Dominic; Coulombe, Kareen L K et al. (2016) Stromal Cells in Dense Collagen Promote Cardiomyocyte and Microvascular Patterning in Engineered Human Heart Tissue. Tissue Eng Part A 22:633-44|
|Kolwicz Jr, Stephen C; Odom, Guy L; Nowakowski, Sarah G et al. (2016) AAV6-mediated Cardiac-specific Overexpression of Ribonucleotide Reductase Enhances Myocardial Contractility. Mol Ther 24:240-50|
|Fernandes, Sarah; Chong, James J H; Paige, Sharon L et al. (2015) Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair. Stem Cell Reports 5:753-62|
|Xing, Yalan; Su, Tin Tin; Ruohola-Baker, Hannele (2015) Tie-mediated signal from apoptotic cells protects stem cells in Drosophila melanogaster. Nat Commun 6:7058|
|Brzezinski, Joseph A; Reh, Thomas A (2015) Photoreceptor cell fate specification in vertebrates. Development 142:3263-73|
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