The purpose of the Bioinformatics Core (Core B) is to provide statistical and bioinformatics analysis of various high-throughput genomics data, primarily from sequencing applications such as ChlP-seq, RNA-seq, Hi-C, ChlA-PET, 4C-seq and replication timing experiments. Each of the three Projects utilize next generation sequencing technologies (NGS) to address questions relating to cell cycle control, chromosome architecture and cell fate decisions in pluripotent stem cells. Our core will provide comprehensive data analysis service to investigators of this Program Project. The PI and supporting staff of this Core have extensive experience in the analysis of high-throughput genomics data, especially NGS data. In addition to basic data analyses, we will work closely with investigators from the three Projects to identify emerging problems that are not addressed sufficiently by existing methods and software. This will involve the development of improved probability models, computer algorithms and analytical strategies to improve data analysis.

Public Health Relevance

The proposed work is relevant to public health because the Core will support research projects that aim to understand the cytological correlation between chromosome structure and the pathogenesis of human diseases. Research devoted to understand the role of chromosome structure in gene regulation also helps the design of strategies for gene therapy.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
2P01GM085354-06
Application #
8641834
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Georgia
Department
Type
DUNS #
City
Athens
State
GA
Country
United States
Zip Code
30322
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Rivera-Mulia, Juan Carlos; Gilbert, David M (2016) Replicating Large Genomes: Divide and Conquer. Mol Cell 62:756-65
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Berger, Ryan P; Sun, Yu Hua; Kulik, Michael et al. (2016) ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells. Stem Cells 34:1742-52
Soufi, Abdenour; Dalton, Stephen (2016) Cycling through developmental decisions: how cell cycle dynamics control pluripotency, differentiation and reprogramming. Development 143:4301-4311

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