Core B will provide a facility for novel optical instrumentation to the investigators of this Program Project. The Core's primary functions will be to assist in experiments that require expertise and specialized equipment not available in the individual sub-project laboratories and to develop state of art optical instrumentation.
The Specific Aims of the Core are to: (1) provide guidance and expert help to the members of the Program Project in using (a) optical trapping to measure force, displacement, and kinetics of motors and adhesion properties of bimolecular interactions, and Total Internal Reflection Microscopy (TIRF), FIONA and other single-molecule fluorescence techniques;and (2) develop (a) combined optical trap-TIRF spectroscopy, (b) methods for measurement of motor stiffness from variance and covariance if single molecule data traces, and (c) techniques for the creation of higher-order motor cytoskeletal assemblies.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM087253-11
Application #
8742371
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-07-31
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
McIntosh, Betsy B; Pyrpassopoulos, Serapion; Holzbaur, Erika L F et al. (2018) Opposing Kinesin and Myosin-I Motors Drive Membrane Deformation and Tubulation along Engineered Cytoskeletal Networks. Curr Biol 28:236-248.e5
Moore, Andrew S; Holzbaur, Erika L F (2018) Mitochondrial-cytoskeletal interactions: dynamic associations that facilitate network function and remodeling. Curr Opin Physiol 3:94-100
Woody, Michael S; Capitanio, Marco; Ostap, E Michael et al. (2018) Electro-optic deflectors deliver advantages over acousto-optical deflectors in a high resolution, ultra-fast force-clamp optical trap. Opt Express 26:11181-11193
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Lippert, Lisa G; Dadosh, Tali; Hadden, Jodi A et al. (2017) Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk. Proc Natl Acad Sci U S A 114:E4564-E4573
Pyrpassopoulos, Serapion; Shuman, Henry; Ostap, E Michael (2017) Adhesion force and attachment lifetime of the KIF16B-PX domain interaction with lipid membranes. Mol Biol Cell 28:3315-3322
Lewis, John H; Jamiolkowski, Ryan M; Woody, Michael S et al. (2017) Deconvolution of Camera Instrument Response Functions. Biophys J 112:1214-1220
Greenberg, Michael J; Shuman, Henry; Ostap, E Michael (2017) Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers. Methods Mol Biol 1486:483-509

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