Core B will provide a facility for novel optical instrumentation to the investigators of this Program Project. The Core's primary functions will be to assist in experiments that require expertise and specialized equipment not available in the individual sub-project laboratories and to develop state of art optical instrumentation.
The Specific Aims of the Core are to: (1) provide guidance and expert help to the members of the Program Project in using (a) optical trapping to measure force, displacement, and kinetics of motors and adhesion properties of bimolecular interactions, and Total Internal Reflection Microscopy (TIRF), FIONA and other single-molecule fluorescence techniques;and (2) develop (a) combined optical trap-TIRF spectroscopy, (b) methods for measurement of motor stiffness from variance and covariance if single molecule data traces, and (c) techniques for the creation of higher-order motor cytoskeletal assemblies.
|Hendricks, Adam G; Goldman, Yale E (2017) Measuring Molecular Forces Using Calibrated Optical Tweezers in Living Cells. Methods Mol Biol 1486:537-552|
|Kast, David J; Dominguez, Roberto (2017) The Cytoskeleton-Autophagy Connection. Curr Biol 27:R318-R326|
|Lippert, Lisa G; Dadosh, Tali; Hadden, Jodi A et al. (2017) Angular measurements of the dynein ring reveal a stepping mechanism dependent on a flexible stalk. Proc Natl Acad Sci U S A 114:E4564-E4573|
|Pyrpassopoulos, Serapion; Shuman, Henry; Ostap, E Michael (2017) Adhesion force and attachment lifetime of the KIF16B-PX domain interaction with lipid membranes. Mol Biol Cell 28:3315-3322|
|Lewis, John H; Jamiolkowski, Ryan M; Woody, Michael S et al. (2017) Deconvolution of Camera Instrument Response Functions. Biophys J 112:1214-1220|
|Greenberg, Michael J; Shuman, Henry; Ostap, E Michael (2017) Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers. Methods Mol Biol 1486:483-509|
|Woody, Michael S; Lewis, John H; Greenberg, Michael J et al. (2016) MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation. Biophys J 111:273-282|
|McIntosh, Betsy B; Ostap, E Michael (2016) Myosin-I molecular motors at a glance. J Cell Sci 129:2689-95|
|Wilson, Meredith H; Bray, Matthew G; Holzbaur, Erika L F (2016) Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells. Methods Mol Biol 1411:269-90|
|Lippert, Lisa G; Hallock, Jeffrey T; Dadosh, Tali et al. (2016) NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching. Bioconjug Chem 27:562-8|
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