Core B will provide a facility for novel optical instrumentation to the investigators of this Program Project. The Core's primary functions will be to assist in experiments that require expertise and specialized equipment not available in the individual sub-project laboratories and to develop state of art optical instrumentation.
The Specific Aims of the Core are to: (1) provide guidance and expert help to the members of the Program Project in using (a) optical trapping to measure force, displacement, and kinetics of motors and adhesion properties of bimolecular interactions, and Total Internal Reflection Microscopy (TIRF), FIONA and other single-molecule fluorescence techniques;and (2) develop (a) combined optical trap-TIRF spectroscopy, (b) methods for measurement of motor stiffness from variance and covariance if single molecule data traces, and (c) techniques for the creation of higher-order motor cytoskeletal assemblies.
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|Wilson, Meredith H; Bray, Matthew G; Holzbaur, Erika L F (2016) Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells. Methods Mol Biol 1411:269-90|
|Moore, Andrew S; Wong, Yvette C; Simpson, Cory L et al. (2016) Dynamic actin cycling through mitochondrial subpopulations locally regulates the fission-fusion balance within mitochondrial networks. Nat Commun 7:12886|
|Lippert, Lisa G; Hallock, Jeffrey T; Dadosh, Tali et al. (2016) NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching. Bioconjug Chem 27:562-8|
|Olenick, Mara A; Tokito, Mariko; Boczkowska, Malgorzata et al. (2016) Hook Adaptors Induce Unidirectional Processive Motility by Enhancing the Dynein-Dynactin Interaction. J Biol Chem 291:18239-51|
|Woody, Michael S; Lewis, John H; Greenberg, Michael J et al. (2016) MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation. Biophys J 111:273-82|
|McIntosh, Betsy B; Ostap, E Michael (2016) Myosin-I molecular motors at a glance. J Cell Sci 129:2689-95|
|Wilson, Meredith H; Holzbaur, Erika L F (2015) Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells. Development 142:218-28|
|Kast, David J; Zajac, Allison L; Holzbaur, Erika L F et al. (2015) WHAMM Directs the Arp2/3 Complex to the ER for Autophagosome Biogenesis through an Actin Comet Tail Mechanism. Curr Biol 25:1791-7|
|Kast, David J; Dominguez, Roberto (2015) WHAMM links actin assembly via the Arp2/3 complex to autophagy. Autophagy 11:1702-4|
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