This Program Project focuses on elucidating the role and actions of novel specialized pro-resolving mediators (SPM) in inflammation and acute tissue injury. In service to this Program Project to achieve its goals, this Core, SPM-Lipidomics and Metabolomics, will provide centralized, standardized and coordinated lipid mediator (LM) lipidomic profiling methods and procedures for Projects 1-4 and Core C. Core B will execute newly established procedures and methods for SPM lipidomics and LM pathway metabolomics as a central service that will be required routinely by Projects 1-4 and Core C. This core will also develop, validate and implement new lipidomic approaches when needed. By providing a centralized location for the LM lipidomics and pathway metabolomics as standardized services, Core B eliminates costly duplication of instruments and personnel within each project. In addition, Core B will characterize the physical properties of synthetic compounds prepared by total organic synthesis in Project 4, and will match these for validation to biologically generated novel SPM, including resolvins, protectins and maresins, before distributing the main bioactive synthetic SPM to each project and Core C for assessing their functional roles. Prioritization ofthe services and selection ofthe specific procedures will be determined through individual meetings with project and core investigators at periodic Program Project group meetings to achieve the optimal use of resources and experience ofthis Core in a cost-effective and timely fashion for the investigators in this Program Project.
Core B will serve Projects 1-4 and play a key role in optimizing resources and standardizing methods for identifying, validating and profiling LM in acute inflammation, tissue injury, and their resolution. By providing these centralized services, Core B will facilitate interactions and coordinate key experiments for the investigators in this Program Project.
|Dalli, Jesmond; Colas, Romain A; Quintana, Carolina et al. (2017) Human Sepsis Eicosanoid and Proresolving Lipid Mediator Temporal Profiles: Correlations With Survival and Clinical Outcomes. Crit Care Med 45:58-68|
|Serhan, Charles N; Chiang, Nan; Dalli, Jesmond (2017) New pro-resolving n-3 mediators bridge resolution of infectious inflammation to tissue regeneration. Mol Aspects Med :|
|English, Justin T; Norris, Paul C; Hodges, Robin R et al. (2017) Identification and Profiling of Specialized Pro-Resolving Mediators in Human Tears by Lipid Mediator Metabolomics. Prostaglandins Leukot Essent Fatty Acids 117:17-27|
|Norris, Paul C; Libreros, Stephania; Chiang, Nan et al. (2017) A cluster of immunoresolvents links coagulation to innate host defense in human blood. Sci Signal 10:|
|Bowden, John A; Heckert, Alan; Ulmer, Candice Z et al. (2017) Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res 58:2275-2288|
|Flitter, Becca A; Hvorecny, Kelli L; Ono, Emiko et al. (2017) Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators. Proc Natl Acad Sci U S A 114:136-141|
|Chiang, Nan; Serhan, Charles N (2017) Structural elucidation and physiologic functions of specialized pro-resolving mediators and their receptors. Mol Aspects Med 58:114-129|
|Fredman, Gabrielle; Spite, Matthew (2017) Specialized pro-resolving mediators in cardiovascular diseases. Mol Aspects Med 58:65-71|
|Hansen, Trond Vidar; Dalli, Jesmond; Serhan, Charles N (2017) The novel lipid mediator PD1n-3 DPA: An overview of the structural elucidation, synthesis, biosynthesis and bioactions. Prostaglandins Other Lipid Mediat 133:103-110|
|Laan, Lisa C; Williams, Andrew R; Stavenhagen, Kathrin et al. (2017) The whipworm (Trichuris suis) secretes prostaglandin E2 to suppress proinflammatory properties in human dendritic cells. FASEB J 31:719-731|
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