Core B The Cell Culture Core, designated as Core B, is a centralized facility that provides primary mouse embryonic fibroblasts, endothelial and epithelial cells isolated from wild-type and genetically modified mice to each of the five projects. Core B also maintains secondary lung cell lines for the PPG and will generate genome-edited cells. Core B will use advanced genome-editing technology to produce cells expressing mEmerald-vimentin, both under the control of its endogenous promoter, as well CRISPR/Cas9-based genome editing to produce cells lacking vimentin protein. The Core personnel have extensive experience in the isolation of primary mouse embryonic fibroblasts, tissue-specific fibroblasts, endothelial, and epithelial cells from mouse tissue (e.g. lung and liver), as well as general cell culture techniques. Centralization of the cell culture facilities will ensure that continuous supply of high-quality cells will be available to each of the five projects. The consolidated cell culture facility provides an economical means of isolating and culturing primary and secondary cells. This translates into reduced overall costs (i.e., personnel, reagents, and animals) and more importantly maintains the quality of the cells used in research. The Core will also provide investigators several murine strains, including vimentinfl/fl mice, which will allow investigators to examine cell-specific roles of genes or pathways using mice expressing Cre recombinase or using tamoxifen-inducible systems. Many of the strains will be used in more than one project, highlighting the synergy and economies of scale achievable by this Program Project. These animals will be used to generate cells and tissues for investigations outlined by the project investigators. In addition, Core B will conduct limited RNA analysis to assess the specific gene expression in vimentin expressing and vimentin-null cells using the Northwestern University Next-Generation Sequencing (NGS) Core Facility. !

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM096971-06A1
Application #
9491496
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2018-09-01
Budget End
2019-03-31
Support Year
6
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Wu, Pei-Hsun; Aroush, Dikla Raz-Ben; Asnacios, Atef et al. (2018) A comparison of methods to assess cell mechanical properties. Nat Methods 15:491-498
Robert, Amélie; Tian, Peirun; Adam, Stephen A et al. (2018) Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport. FASEB J :fj201800604R
Li, Wen; Zhang, Liyuan; Ge, Xuehui et al. (2018) Microfluidic fabrication of microparticles for biomedical applications. Chem Soc Rev 47:5646-5683
Wang, Zheng; Divanyan, Alex; Jourd'heuil, Frances L et al. (2018) Vimentin expression is required for the development of EMT-related renal fibrosis following unilateral ureteral obstruction in mice. Am J Physiol Renal Physiol 315:F769-F780
Wang, Liqun; Xia, Jing; Li, Jonathan et al. (2018) Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease. Nat Commun 9:1899
Bucki, Robert; Durna?, Bonita; W?tek, Marzena et al. (2018) Targeting polyelectrolyte networks in purulent body fluids to modulate bactericidal properties of some antibiotics. Infect Drug Resist 11:77-86
Zaritsky, Assaf; Obolski, Uri; Gan, Zhuo et al. (2017) Decoupling global biases and local interactions between cell biological variables. Elife 6:
Roudot, Philippe; Liya Ding; Jaqaman, Khuloud et al. (2017) Piecewise-Stationary Motion Modeling and Iterative Smoothing to Track Heterogeneous Particle Motions in Dense Environments. IEEE Trans Image Process 26:5395-5410
Barlan, Kari; Gelfand, Vladimir I (2017) Microtubule-Based Transport and the Distribution, Tethering, and Organization of Organelles. Cold Spring Harb Perspect Biol 9:
Guo, Ming; Pegoraro, Adrian F; Mao, Angelo et al. (2017) Cell volume change through water efflux impacts cell stiffness and stem cell fate. Proc Natl Acad Sci U S A 114:E8618-E8627

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