The study of mutagenesis, the theme that unites this Program Project, involves intensive analysis of DNA for the nature and frequency of mutations in particular genomic targets. This typically involves sets in the hundreds of isolates. The execution of such experiments and the analysis of data derived from these is costly and time-consuming. The determination of mutational spectra that are intrinsic to the projects demands two types of analyses: accurate DNA fragment length measurements and DNA sequencing. There is a need in the field for detection of mutations in a larger genomic context, to expand the regions that can be surveyed and to detect the types of mutations that have traditionally posed difficulties for molecular analysis, such as large genomic rearrangements. Highly parallel DNA sequencing platforms now provide an opportunity to extend the scope of mutational analysis. Common needs for the Project are DNA analyses that are convenient and high-throughput, both in physical execution and data analysis.The overall goal of this Core is to provide facilities in which state-of-the-art sequence and fragment length analysis can be coordinated, in cost-effective ways and at a scale and speed demanded by the needs of the program project investigators. A key feature of the Core will be protocoldevelopment, user training, centralized quality control, and data analysis tools, centralized on a shared Wiki site and supported through group interactions. Bioinformatic support will be provided to allow investigators to develop appropriate analysis for their unique needs. The establishment of these facilities will maximize resources, promote development and sharing of expertise and will improve the efficiency of each project.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Program Projects (P01)
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Special Emphasis Panel (ZRG1-GGG-Q (40))
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Brandeis University
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Beagan, Kelly; McVey, Mitch (2016) Linking DNA polymerase theta structure and function in health and disease. Cell Mol Life Sci 73:603-15
Rodgers, Kasey; McVey, Mitch (2016) Error-Prone Repair of DNA Double-Strand Breaks. J Cell Physiol 231:15-24
Aksenova, Anna Y; Han, Gil; Shishkin, Alexander A et al. (2015) Expansion of Interstitial Telomeric Sequences in Yeast. Cell Rep 13:1545-51
Shah, Kartik A; Mirkin, Sergei M (2015) The hidden side of unstable DNA repeats: Mutagenesis at a distance. DNA Repair (Amst) 32:106-12
Kloosterman, Wigard P; Francioli, Laurent C; Hormozdiari, Fereydoun et al. (2015) Characteristics of de novo structural changes in the human genome. Genome Res 25:792-801
Haber, James E (2015) TOPping off meiosis. Mol Cell 57:577-81
Usdin, Karen; House, Nealia C M; Freudenreich, Catherine H (2015) Repeat instability during DNA repair: Insights from model systems. Crit Rev Biochem Mol Biol 50:142-67
Pandey, Shristi; Ogloblina, Anna M; Belotserkovskii, Boris P et al. (2015) Transcription blockage by stable H-DNA analogs in vitro. Nucleic Acids Res 43:6994-7004
Su, Xiaofeng A; Dion, Vincent; Gasser, Susan M et al. (2015) Regulation of recombination at yeast nuclear pores controls repair and triplet repeat stability. Genes Dev 29:1006-17
House, Nealia C M; Yang, Jiahui H; Walsh, Stephen C et al. (2014) NuA4 initiates dynamic histone H4 acetylation to promote high-fidelity sister chromatid recombination at postreplication gaps. Mol Cell 55:818-28

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