A. Introduction. Pregnant sheep will continue to be used for these studies, as they are readily available, are relatively inexpensive (as compared with primates), the fetuses are large enough so that adequate amounts of the several tissues can be studied (cerebral, uterine, and pulmonary arteries, adrenal glands, ef cetera), and we and others have amassed a considerable body of physiologic data on this species. Essentially all of the individual projects in this proposal will use pregnant or nonpregnant sheep which have been acclimatized to high altitude, long-term hypoxemia, or normoxic controls. At the time of sacrifice, tissues from these animals will be partitioned among the several investigators and projects of this proposal, as well as several other investigators. Thus, this Technical Core includes the animals for these studies. In addition, it includes the expense for several major items of equipment required for most of these experiments. The experimental animal and equipment Technical Core will be utilized for essentially every project in common by all members of the Center. Such a Technical Core optimizes the efficient operation of the laboratories, the experiments per se, and the data acquisition and analysis. The essential features of the experimental animal procurement and laboratory are detailed below. Following a general description of our laboratories, equipment, and laboratory animal facilities, the Technical Core will be considered from several aspects: Experimental Animals, White Mountain Research Station, LLU Animal Facilities, Laboratory Core Facilities, and Budget. Drs. Longo and Ducsay will devote 10% of their time to administer the Technical Core. The principal investigators will continue to share major items of equipment including: imaging systems, ultracentrifuges, centrifuges, radioactivity counters, recorders, blood-gas analyzers, analyzers for Western immunoblot, realtime PCR, confocal microscopy, freezers, and other items. In addition, we will continue to share the experimental animals, and surgical operating theater. This fiscally efficient arrangement, together with the scientific collaboration among the investigators js one of the reasons that broad based research support is sought.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD031226-20
Application #
8704968
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
20
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Loma Linda University
Department
Type
DUNS #
City
Loma Linda
State
CA
Country
United States
Zip Code
92354
Vrancken, Kurt; Schroeder, Hobe J; Longo, Lawrence D et al. (2016) Postprandial lipids accelerate and redirect nitric oxide consumption in plasma. Nitric Oxide 55-56:70-81
Liu, Taiming; Schroeder, Hobe J; Wilson, Sean M et al. (2016) Local and systemic vasodilatory effects of low molecular weight S-nitrosothiols. Free Radic Biol Med 91:215-23
Blum-Johnston, Carla; Thorpe, Richard B; Wee, Chelsea et al. (2016) Developmental acceleration of bradykinin-dependent relaxation by prenatal chronic hypoxia impedes normal development after birth. Am J Physiol Lung Cell Mol Physiol 310:L271-86
Hu, Xiang-Qun; Huang, Xiaohui; Xiao, Daliao et al. (2016) Direct effect of chronic hypoxia in suppressing large conductance Ca(2+)-activated K(+) channel activity in ovine uterine arteries via increasing oxidative stress. J Physiol 594:343-56
Myers, Dean A; Singleton, Krista; Kenkel, Christy et al. (2016) Gestational hypoxia modulates expression of corticotropin-releasing hormone and arginine vasopressin in the paraventricular nucleus in the ovine fetus. Physiol Rep 4:
Mata-Greenwood, Eugenia; Jackson, P Naomi; Pearce, William J et al. (2015) Endothelial glucocorticoid receptor promoter methylation according to dexamethasone sensitivity. J Mol Endocrinol 55:133-46
Newby, Elizabeth A; Kaushal, Kanchan M; Myers, Dean A et al. (2015) Adrenocorticotropic Hormone and PI3K/Akt Inhibition Reduce eNOS Phosphorylation and Increase Cortisol Biosynthesis in Long-Term Hypoxic Ovine Fetal Adrenal Cortical Cells. Reprod Sci 22:932-41
Chuang, Tsai-Der; Pearce, William J; Khorram, Omid (2015) miR-29c induction contributes to downregulation of vascular extracellular matrix proteins by glucocorticoids. Am J Physiol Cell Physiol 309:C117-25
Adeoye, Olayemi O; Silpanisong, Jinjutha; Williams, James M et al. (2015) Role of the sympathetic autonomic nervous system in hypoxic remodeling of the fetal cerebral vasculature. J Cardiovasc Pharmacol 65:308-16
Myers, Dean A; Singleton, Krista; Hyatt, Kim et al. (2015) Long-Term Gestational Hypoxia Modulates Expression of Key Genes Governing Mitochondrial Function in the Perirenal Adipose of the Late Gestation Sheep Fetus. Reprod Sci 22:654-63

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