A cardinal feature of the program project Is Its Integration of brainstem physiology and neuroanatomy at the regional, cellular, and subcellular levels as It relates to normal and abnormal homeostasis. Core B, the Neuroanatomy Core, provides high-quality, state-of-the-art neuroanatomy services to each of the individual projects, thereby facilitating completion of the program's Integrative specific alms. It Is a central resource that ensures consistent study design and execution In the neuroanatomic studies of the program project. Its centralization streamlines technical procedures, eliminates potential duplication, and facilitates Integration of neuroanatomic approaches across the projects. The core also facilitates the comparison and cross- fertilization between studies of human neuropathology and animal models. The Neuroanatomy Core B will continue to be based In the Department of Anesthesiology, Critical Care and Pain Medicine, Children's Hospital Boston, and to be directed by Dr. Kathryn Commons. She has a background in serotonin neurobiology and neuroanatomy, and the effects of nicotine upon serotonergic mechanisms, subjects germane to SIDS and the proposed specific alms of the program. Staffing Includes a highly expert and productive staff scientist. Core B resources Include the microscopy and technical resources of Dr. Commons'laboratory, supplemented by a network of supporting common resources at the division, department, hospital and university-wide level. Core B services Include consultations regarding optimal neuroanatomic procedures for individual specific aims, training In methodological approaches and techniques, and direct participation in the neuroanatomic aspects of Individual projects. Methods available in Core B include quantitative tissue receptor autoradiography, array tomography, multiple Immunolabeling, anterograde and retrograde tract-tracing, combined tract-tracing and Immunolabeling, quantitation and imaging of axonal terminals, and in situ hybridization. Core B also assists with refining study design, data summary and Interpretation and manuscript preparation. The core has a proven track record In neuroanatomic contributions to date In the current cycle, with several resulting publications, abstracts, and manuscripts in preparation. This progress represents a contribution to all of the individual projects. Furthermore, Core B has contributed to studies that span more than one project, thereby contributing to the active collaboration and synergism that characterizes this program project as a whole.

Public Health Relevance

The mission of the program project Is to elucidate the role of the brainstem and Interconnected brain regions in the sudden Infant death syndrome (SIDS), the leading cause of postneonatal infant mortality In the United States today. Neuroanatomy Core B provides a resource for state-of-the-art neuroanatomic services to the four projects of the program.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZHD1-DSR-Z)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Children's Hospital Boston
United States
Zip Code
Rognum, Ingvar J; Tran, Hoa; Haas, Elisabeth A et al. (2014) Serotonin metabolites in the cerebrospinal fluid in sudden infant death syndrome. J Neuropathol Exp Neurol 73:115-22
Espinosa-Medina, I; Outin, E; Picard, C A et al. (2014) Neurodevelopment. Parasympathetic ganglia derive from Schwann cell precursors. Science 345:87-90
Cerpa, V; Gonzalez, A; Richerson, G B (2014) Diphtheria toxin treatment of Pet-1-Cre floxed diphtheria toxin receptor mice disrupts thermoregulation without affecting respiratory chemoreception. Neuroscience 279:65-76
Corcoran, Andrea E; Commons, Kathryn G; Wu, Yuanming et al. (2014) Dual effects of 5-HT(1a) receptor activation on breathing in neonatal mice. J Neurosci 34:51-9
Jensen, Patricia; Dymecki, Susan M (2014) Essentials of recombinase-based genetic fate mapping in mice. Methods Mol Biol 1092:437-54
Ray, Russell S; Corcoran, Andrea E; Brust, Rachael D et al. (2013) Egr2-neurons control the adult respiratory response to hypercapnia. Brain Res 1511:115-25
Xia, L; Leiter, J C; Bartlett Jr, D (2013) Laryngeal reflex apnea in neonates: effects of CO2 and the complex influence of hypoxia. Respir Physiol Neurobiol 186:109-13
Paterson, David S (2013) Serotonin gene variants are unlikely to play a significant role in the pathogenesis of the sudden infant death syndrome. Respir Physiol Neurobiol 189:301-14
Corcoran, Andrea E; Richerson, George B; Harris, Michael B (2013) Serotonergic mechanisms are necessary for central respiratory chemoresponsiveness in situ. Respir Physiol Neurobiol 186:214-20
Arnal, Ashley V; Gore, Julie L; Rudkin, Alison et al. (2013) Influence of age, body temperature, GABAA receptor inhibition and caffeine on the Hering-Breuer inflation reflex in unanesthetized rat pups. Respir Physiol Neurobiol 186:73-80

Showing the most recent 10 out of 97 publications