This core provides adenovirus vectors for both in vitro and in vivo studies. Intraductal delivery resulting in transduction of mammary epithelial cells in the intact gland in the absence of inflammation has been extensively developed [26]. Although the majority of the experimental work that went into developing this system was conducted in the Neville laboratory, the McManaman laboratory has made extensive use of this technology during the present funding period. In the new proposal, all projects of plan to make use of this technology. A full list of the viruses currently available is presented in the Core Description.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
5P01HD038129-14
Application #
8703146
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Type
DUNS #
City
Aurora
State
CO
Country
United States
Zip Code
80045
Wellberg, Elizabeth A; Anderson, Steven M (2014) FASNating targets of metformin in breast cancer stem-like cells. Horm Cancer 5:358-62
Schmidt, Jeffrey W; Wehde, Barbara L; Sakamoto, Kazuhito et al. (2014) Stat5 regulates the phosphatidylinositol 3-kinase/Akt1 pathway during mammary gland development and tumorigenesis. Mol Cell Biol 34:1363-77
Rudolph, Michael C; Wellberg, Elizabeth A; Lewis, Andrew S et al. (2014) Thyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium. J Lipid Res 55:1052-1065
Pereira, Renata O; Wende, Adam R; Crum, Ashley et al. (2014) Maintaining PGC-1? expression following pressure overload-induced cardiac hypertrophy preserves angiogenesis but not contractile or mitochondrial function. FASEB J 28:3691-702
McManaman, James L (2014) Lipid transport in the lactating mammary gland. J Mammary Gland Biol Neoplasia 19:35-42
Saben, Jessica L; Bales, Elise S; Jackman, Matthew R et al. (2014) Maternal obesity reduces milk lipid production in lactating mice by inhibiting acetyl-CoA carboxylase and impairing fatty acid synthesis. PLoS One 9:e98066
Obr, Alison E; Grimm, Sandra L; Bishop, Kathleen A et al. (2013) Progesterone receptor and Stat5 signaling cross talk through RANKL in mammary epithelial cells. Mol Endocrinol 27:1808-24
Neville, Margaret C; Webb, Patricia; Ramanathan, Palaniappan et al. (2013) The insulin receptor plays an important role in secretory differentiation in the mammary gland. Am J Physiol Endocrinol Metab 305:E1103-14
Webb, Patricia G; Spillman, Monique A; Baumgartner, Heidi K (2013) Claudins play a role in normal and tumor cell motility. BMC Cell Biol 14:19
Pereira, Renata O; Wende, Adam R; Olsen, Curtis et al. (2013) Inducible overexpression of GLUT1 prevents mitochondrial dysfunction and attenuates structural remodeling in pressure overload but does not prevent left ventricular dysfunction. J Am Heart Assoc 2:e000301

Showing the most recent 10 out of 73 publications