The maternal-fetal interface is critically important since is at this level that uteroplacental and fetoplacental blood flows are regulated and dysfunctions are seen in diseases of pregnancy, e.g. preeclampsia. Vasodilation is controlled by PGI2 and Nitric Oxide (NO) of endothelial origin and locally shows obligatory requirements for cell-cell communication via Gap junctions, a common theme in this PO-1. Uterine Artery Endothelial Cells in Pregnancy (P-UAEC) and HUVECs undergo """"""""Programmed Adaptation"""""""" to maintain NO production via a Connexin (Cx) 43 mediated mechanisms. HYPOTHESIS Aim 1: Endothelial """"""""Programmed Adaptation"""""""" is modulated by the Gap junction protein Cx43 that is elevated directly by Shear Stress via cAMP and/or cGMP to maintain vasodilator (PGI2 and NO) production. Purpose Aim 1: To determine in P-UAECs, HUVECs and HUAECs the effects of: 1) acute vs. prolonged Laminar Shear Stress on levels of phosphorylated and total Cx43 &eNOS and vasodilator production (PGI2 and NO) relative to cyclic nucleotide (cAMP and cGMP) adaptation;2) inhibition of PKA, PKG, Adenylate Cyclase and sol Guanylate Cyclase;and 3) Indomethacin and L-NAME on these shear stress responses. HYPOTHESIS Aim 2: These important shear stress-mediated vasodilator mechanisms are abrogated by patho-physiologic levels of representative cytokines elevated in preeclampsia [VEGF and Tumor Necrosis Factor] via functional Gap junction disruption. We hypothesize that shear stress elevates cAMP /cGMP which is """"""""protective"""""""" for maintaining vasodilator production via Gap junction assembly. Purpose Aim 2: To determine in P-UAECs, HUVECs and HUAECs if these cytokines disrupt the shear stress-associated cyclic nucleotide Gap junction-mediated vasodilator mechanisms we will evaluate: 1) effects of treatment with VEGF and TNF on the acute and prolonged laminar shear stress responses;2) the """"""""therapeutic protective"""""""" effects 8-Br-cAMP and 8-Br-cGMP on VEGF and TNF disruption of shear stress responses;and 3) the """"""""therapeutic protective"""""""" effects cAMP phosphodiesterase (PDE)2, cAMP PDES, cAMP PDE4, or cGMP PDE5 inhibitors on VEGF and TNF disruption of shear stress responses. These data provide the first mechanistic framework for understanding the interactions between shear stress-mediated cyclic nucleotides for """"""""therapeutic protective actions"""""""" and Gap junctions to regulate endothelial vasodilator (PGI2 &NO) production and it's abrogation via cytokine (VEGF &TNF) mediated disruption of cell-cell communication.
These studies represent major advancements in understanding the protective (therapeutic) effects of shear stress mediators on molecular/signaling mechanisms regulating uterine and placental endothelial adaptation via Gap junction-mediated cell-cell communication at the maternal-fetal interface. These mechanisms underlying uterine and placental endothelial vasodilator (PGI2 and NO) dysfunction caused by cytokines (VEGF and TNF) in preeclampsia and their abrogation by shear stress induced cAMP/cGMP.
|Schreier, David A; Forouzan, Omid; Hacker, Timothy A et al. (2016) Increased Red Blood Cell Stiffness Increases Pulmonary Vascular Resistance and Pulmonary Arterial Pressure. J Biomech Eng 138:021012|
|Ampey, Bryan C; Morschauser, Timothy J; Ramadoss, Jayanth et al. (2016) Domain-Specific Partitioning of Uterine Artery Endothelial Connexin43 and Caveolin-1. Hypertension 68:982-8|
|Pastore, Mayra B; Talwar, Saira; Conley, Meghan R et al. (2016) Identification of Differential ER-Alpha Versus ER-Beta Mediated Activation of eNOS in Ovine Uterine Artery Endothelial Cells. Biol Reprod 94:139|
|Rozner, Ann E; Durning, Maureen; Kropp, Jenna et al. (2016) Macrophages modulate the growth and differentiation of rhesus monkey embryonic trophoblasts. Am J Reprod Immunol 76:364-375|
|Li, Yan; Wang, Kai; Zou, Qing-Yun et al. (2015) 2,3,7,8-Tetrachlorodibenzo-p-dioxin differentially suppresses angiogenic responses in human placental vein and artery endothelial cells. Toxicology 336:70-8|
|Morris, Erin A; Hale, Sarah A; Badger, Gary J et al. (2015) Pregnancy induces persistent changes in vascular compliance in primiparous women. Am J Obstet Gynecol 212:633.e1-6|
|Boeldt, Derek S; Grummer, Mary A; Yi, FuXian et al. (2015) Phosphorylation of Ser-279/282 and Tyr-265 positions on Cx43 as possible mediators of VEGF-165 inhibition of pregnancy-adapted Ca2+ burst function in ovine uterine artery endothelial cells. Mol Cell Endocrinol 412:73-84|
|Li, Yan; Wang, Kai; Zou, Qing-Yun et al. (2015) A possible role of aryl hydrocarbon receptor in spontaneous preterm birth. Med Hypotheses 84:494-7|
|Ampey, Bryan; Bird, Ian; Magness, Ron (2015) [307-POS]: Cx43 phosphorylation and the functionality of Cx43 gap junctions are moderated by cyclic nucleotide activity in UAECs and HUVECs. Pregnancy Hypertens 5:151-2|
|Anaya, Heather A; Yi, Fu-Xian; Boeldt, Derek S et al. (2015) Changes in Ca2+ Signaling and Nitric Oxide Output by Human Umbilical Vein Endothelium in Diabetic and Gestational Diabetic Pregnancies. Biol Reprod 93:60|
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