In the context of preparing for this upcoming Program Project Grant (POl) renewal, over the last five years we have developed novel and cutting edge imaging methodology and established advanced protein analytical methods related to 0x43 analysis, as well as further training an expanding group of project personnel in general Ca2+ imaging and 0x43 protein analysis. We have also developed considerable expertise in dual Ca2+/N0 detection in endothelial cells of intact vessels and all these methods are now increasingly central to the projects of this combined POl. In the current application, continued training of current and new personnel and ongoing technical support of imaging methods application will continue through this new Core B and ongoing instrument technical support will be added for imaging microscopy. In addition, the Core will provide training support for cell protein analysis by Western blot based and Co-IP procedures as well as new antibody and technical assay development for all four projects. As a coordinating unit, the Core will offer further support to enhance data collection uniformity by relieving project personnel of the burden of preparing frozen stocks of validated pooled endothelial cells for imaging, so ensuring both high qualities of cell preparations providing uniformity of data acquisition between the projects. Where opportunities for reagent purchase can improve cost savings, or at least ensure more abundant reagents are fully used, the Core will coordinate those supplies to secure savings.
of the Core is to support methods of live cell imaging and protein analysis as central to multiple projects. The staff core are those who have developed the methods and will train other Project Staff. The Core will also support maintenance and calibration of instruments for live cell imaging and support current methods application and new methods development in live cell imaging and cell protein analysis.
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|Ampey, Bryan C; Ampey, Amanda C; Lopez, Gladys E et al. (2017) Cyclic Nucleotides Differentially Regulate Cx43 Gap Junction Function in Uterine Artery Endothelial Cells From Pregnant Ewes. Hypertension 70:401-411|
|Li, Yan; Wang, Kai; Zou, Qing-Yun et al. (2017) ITE Suppresses Angiogenic Responses in Human Artery and Vein Endothelial Cells: Differential Roles of AhR. Reprod Toxicol 74:181-188|
|Landeros, Rosalina Villalon; Jobe, Sheikh O; Aranda-Pino, Gabrielle et al. (2017) Convergent ERK1/2, p38 and JNK mitogen activated protein kinases (MAPKs) signalling mediate catecholoestradiol-induced proliferation of ovine uterine artery endothelial cells. J Physiol 595:4663-4676|
|Degner, Kenna; Magness, Ronald R; Shah, Dinesh M (2017) Establishment of the Human Uteroplacental Circulation: A Historical Perspective. Reprod Sci 24:753-761|
|Boeldt, D S; Bird, I M (2017) Vascular adaptation in pregnancy and endothelial dysfunction in preeclampsia. J Endocrinol 232:R27-R44|
|Rozner, Ann E; Durning, Maureen; Kropp, Jenna et al. (2016) Macrophages modulate the growth and differentiation of rhesus monkey embryonic trophoblasts. Am J Reprod Immunol 76:364-375|
|Pastore, Mayra B; Talwar, Saira; Conley, Meghan R et al. (2016) Identification of Differential ER-Alpha Versus ER-Beta Mediated Activation of eNOS in Ovine Uterine Artery Endothelial Cells. Biol Reprod 94:139|
|Schreier, David A; Forouzan, Omid; Hacker, Timothy A et al. (2016) Increased Red Blood Cell Stiffness Increases Pulmonary Vascular Resistance and Pulmonary Arterial Pressure. J Biomech Eng 138:021012|
|Ampey, Bryan C; Morschauser, Timothy J; Ramadoss, Jayanth et al. (2016) Domain-Specific Partitioning of Uterine Artery Endothelial Connexin43 and Caveolin-1. Hypertension 68:982-8|
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