The main benefit of this Core facility to the Program Project is that it provides animals with uniform surgical and molecular manipulations to all the Projects tnat require them. Thus, animals will be treated identically from the time of their mothers' arrival to the facility to the time they are shipped to various Projects. In addition, the neurons manipulated in these animals will have been done so with the same preparations of shRNA and expression plasmids. This is important not only from an anatomic perspective where it is hoped that inter-individual variability in injection size and location is minimized but also from the point of view of the developmental history of the animal, an especially important factor for animals undergoing behavioral testing. Live animals will be used by Projects I and IH, and perfused brains will be snipped to Project II. The Core has been successfully performing these in utero electtoporations surgeries and shRNA design and use for 7 years.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD057853-04
Application #
8376374
Study Section
Special Emphasis Panel (ZHD1-MRG-C)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
4
Fiscal Year
2012
Total Cost
$202,683
Indirect Cost
$40,582
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
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