PROJECT 3 This project's goal is to resolve the sequence structure of 20 complex genomic regions where common genome structural variation is associated with disease. In collaboration with Chris Amemiya, we propose to construct large-insert clone (BAC) libraries (10-fold coverage) from 18 reference genomes from the 1000 Genomes Project. We will target regions and individuals identified from Projects 1 and 2, retrieve and characterize BACs, and use next-generation sequencing (NGS) lllumina clone pooling strategy sequence to resolve haplotypes of these regions. As part of our collaboration with Washington University Genome Sequencing Center (WUGSC), we will select haplotype-resolved tiling paths for complete high-quality sequencing. The results of this work will provide common high-quality alternate haplotypes of biomedical relevance to be integrated into the human reference genome.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Program Projects (P01)
Project #
5P01HG004120-07
Application #
8489318
Study Section
Ethical, Legal, Social Implications Review Committee (GNOM)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
7
Fiscal Year
2013
Total Cost
$176,820
Indirect Cost
$57,152
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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