We have considerable experience at the DMA manipulation, tissue culture, protein purification, and protein characterization required to produce Gla containing and other proteins [1-6]. Specifically we have experience with the techniques required to assess posttranslational modification, including carboxylation, of the Gla containing coagulation proteins [1,7,8]. Protein production in the Core will be done by a dedicated technician. Protein purification and characterization will be done by a dedicated technician and by the Director.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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University of North Carolina Chapel Hill
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Bode, Michael; Mackman, Nigel (2014) Regulation of tissue factor gene expression in monocytes and endothelial cells: Thromboxane A2 as a new player. Vascul Pharmacol 62:57-62
Parker, Christine H; Morgan, Christopher R; Rand, Kasper D et al. (2014) A conformational investigation of propeptide binding to the integral membrane protein ?-glutamyl carboxylase using nanodisc hydrogen exchange mass spectrometry. Biochemistry 53:1511-20
Feng, D; Stafford, K A; Broze, G J et al. (2013) Evidence of clinically significant extravascular stores of factor IX. J Thromb Haemost 11:2176-8
Owens 3rd, A Phillip; Mackman, Nigel (2012) Sources of tissue factor that contribute to thrombosis after rupture of an atherosclerotic plaque. Thromb Res 129 Suppl 2:S30-3
Owens 3rd, A Phillip; Passam, Freda H; Antoniak, Silvio et al. (2012) Monocyte tissue factor-dependent activation of coagulation in hypercholesterolemic mice and monkeys is inhibited by simvastatin. J Clin Invest 122:558-68
Wu, Sangwook; Liu, Shubin; Davis, Charles H et al. (2011) A hetero-dimer model for concerted action of vitamin K carboxylase and vitamin K reductase in vitamin K cycle. J Theor Biol 279:143-9
Owens 3rd, A Phillip; Mackman, Nigel (2011) Microparticles in hemostasis and thrombosis. Circ Res 108:1284-97
Monroe, Dougald M; Mackman, Nigel; Hoffman, Maureane (2010) Wound healing in hemophilia B mice and low tissue factor mice. Thromb Res 125 Suppl 1:S74-7
Mackman, Nigel; Becker, Richard C (2010) DVT: a new era in anticoagulant therapy. Arterioscler Thromb Vasc Biol 30:369-71
de Courcy, B; Pedersen, L G; Parisel, O et al. (2010) Understanding selectivity of hard and soft metal cations within biological systems using the subvalence concept. I. Application to blood coagulation: direct cation-protein electronic effects vs. indirect interactions through water networks. J Chem Theory Comput 6:1048-1063

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