The Animal Models Core will make several types of animals for the PPG investigators. It will make several transgenic constructs. These transgenic constructs will be used to make a number of mouse models: MsrA condifional transgene, nAchR over expression transgene, NADPH oxidase knock down, and an MrsA knockdown. It will also produce a number of vectors for gene ablations in both rats and mice. Gene knockouts in rats include AT1 and a7 nAchR. A gene-targeting vector for a nAChR knockout mouse will be made if KOMP vectors and ES cell clones are not available. An nAChR null mouse will be produced. Some projects require involved breeding schemes which will be conducted under the Core's direcfion in an SPF barrier facility.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
2P01HL014388-41A1
Application #
8705087
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
41
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Iowa
Department
Type
DUNS #
City
Iowa City
State
IA
Country
United States
Zip Code
52242
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