The Animal Core continues to provide the fundamental support to all PPG investigators in terms of providing services for hemodynamic measurements in rats, mice and calves. This includes mean, systolic and diastolic blood pressures in the pulmonary and systemic vasculature, cardiac outputs and right/ left ventricular function. The Core provides the expertise to measure these parameters in the anesthetized and awake animal models with various systems including the Millar catheter system, echocardiography, pressure filled transducers, and dye dilution method for cardiac output measurements. By providing a wide range of options for hemodynamic measurements the core insures that the individual experimental design can be matched to the appropriate data collection system encompassing all of investigators needs. In addition to hemodynamic services the Core also maintains and provides assistance with use of the hyperand hypobaric chamber facility. Historically, these chambers have been used for the study of pulmonary hypertension. Notably, The facility has recently expanded in size to ~400 sq ft. The hypobaric chambers are depressurized by individual vacuum pumps housed in a separate area. All chambers are equipped with automatic devices to return to ambient conditions in case of power failure. The hyperbaric chambers are pressurized by filtered compressed air and typically used to maintain animals at sea level pressure (760 mmHg). Besides providing technical (hemodynamic) and methodological (chambers) support the Core also assists with maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. This includes maintenance of breeding colonies for inbred mice and generation of tissue-specific knockout models. The Core will assist investigators with the breeding, genotyping, and maintenance of these animal colonies as needed. In addition, the Animal Core will be involved in the generation of targeted knockout models. Specific to this proposal, investigators of this PPG project are generating inducible smooth musclespecific PTEN, PPARv, and CREB knockout mice

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL014985-40
Application #
8502291
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
40
Fiscal Year
2013
Total Cost
$271,498
Indirect Cost
$93,480
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Cohrs, Randall J; Lee, Katherine S; Beach, Addilynn et al. (2017) Targeted Genome Sequencing Reveals Varicella-Zoster Virus Open Reading Frame 12 Deletion. J Virol 91:
Lapel, Martin; Weston, Philip; Strassheim, Derek et al. (2017) Glycolysis and oxidative phosphorylation are essential for purinergic receptor-mediated angiogenic responses in vasa vasorum endothelial cells. Am J Physiol Cell Physiol 312:C56-C70
Lin, Y-C; Sung, Y K; Jiang, X et al. (2017) Simultaneously Targeting Myofibroblast Contractility and Extracellular Matrix Cross-Linking as a Therapeutic Concept in Airway Fibrosis. Am J Transplant 17:1229-1241
Tuder, Rubin M (2017) Pulmonary vascular remodeling in pulmonary hypertension. Cell Tissue Res 367:643-649
Loomis, Zoe; Eigenberger, Paul; Redinius, Katherine et al. (2017) Correction: Hemoglobin induced cell trauma indirectly influences endothelial TLR9 activity resulting in pulmonary vascular smooth muscle cell activation. PLoS One 12:e0173652
Murakami, A; Wang, L; Kalhorn, S et al. (2017) Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis 6:e287
de Bourcy, Charles F A; Dekker, Cornelia L; Davis, Mark M et al. (2017) Dynamics of the human antibody repertoire after B cell depletion in systemic sclerosis. Sci Immunol 2:
Cree-Green, Melanie; Gupta, Abhinav; Coe, Gregory V et al. (2017) Insulin resistance in type 2 diabetes youth relates to serum free fatty acids and muscle mitochondrial dysfunction. J Diabetes Complications 31:141-148
Jansing, Nicole L; McClendon, Jazalle; Henson, Peter M et al. (2017) Unbiased Quantitation of Alveolar Type II to Alveolar Type I Cell Transdifferentiation during Repair after Lung Injury in Mice. Am J Respir Cell Mol Biol 57:519-526
Loomis, Zoe; Eigenberger, Paul; Redinius, Katherine et al. (2017) Hemoglobin induced cell trauma indirectly influences endothelial TLR9 activity resulting in pulmonary vascular smooth muscle cell activation. PLoS One 12:e0171219

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