The Microscopy and Image Analysis Core, which serves all investigators in the Program Project, consolidates tissue preparation, microscopy, and image analysis in the same laboratory, under the direction of staff with appropriate expertise. The laboratory provides investigators and their associates with the expertise, equipment, supplies, and, when appropriate, the training needed for morphologic aspects of their research. The Core Facility is located within the laboratory space at the Laurel Heights Campus. The equipment in the Facility includes: an electron microscope, cryostat, ultramicrotome, immunofluorescence microscope, confocal microscopic analysis system, digital and film cameras, image analysis systems, and a fully equipped photographic darkroom (important for electron microscopy). The Core facility has been in continuous operation since 1994. This facility ensures that specialized equipment is always available in good working order and technical expertise is readily available. The Core Facility as currently constituted enhances the ability of the Core staff to provide individual attention to the particular problems involved in individual projects. It has been critical to the performance of morphologic analyses among the individual projects of the Program Project Grant.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL024075-34
Application #
8603263
Study Section
Special Emphasis Panel (ZHL1-PPG-S)
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
34
Fiscal Year
2014
Total Cost
$185,285
Indirect Cost
$65,146
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Barrette, Anne Marie; Roberts, Jessica K; Chapin, Cheryl et al. (2016) Antiinflammatory Effects of Budesonide in Human Fetal Lung. Am J Respir Cell Mol Biol 55:623-632
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Gonzales, Linda W; Gonzalez, Robert; Barrette, Anne Marie et al. (2015) Expression of Carcinoembryonic Cell Adhesion Molecule 6 and Alveolar Epithelial Cell Markers in Lungs of Human Infants with Chronic Lung Disease. J Histochem Cytochem 63:908-21
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Raymond, Wilfred W; Xu, Xiang; Nimishakavi, Shilpa et al. (2015) Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS One 10:e0125797
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Chapin, Cheryl; Bailey, Nicole A; Gonzales, Linda W et al. (2012) Distribution and surfactant association of carcinoembryonic cell adhesion molecule 6 in human lung. Am J Physiol Lung Cell Mol Physiol 302:L216-25
Heine, Vivi M; Griveau, Amelie; Chapin, Cheryl et al. (2011) A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Sci Transl Med 3:105ra104
Gonzalez, Robert F; Allen, Lennell; Gonzales, Linda et al. (2010) HTII-280, a biomarker specific to the apical plasma membrane of human lung alveolar type II cells. J Histochem Cytochem 58:891-901

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