The major objective of the Mouse Core is to provide a centralized facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. All three projects in this proposal will utilize transgenic approaches in their studies and will participate in the Core. The continuation of this Core will result in economy of effort and expense by avoiding duplication of a highly technical skill requiring specialized equipment. The Core will also facilitate collaborations between projects. Dedicated space and equipment for microinjection of eggs and blastocysts has been secured on the Parnassus Campus and adequate animal space is assigned to the Principal Investigator for Core functions. Most of the mouse lines described in the Progress Report and Preliminary Data sections of the proposal have been generated using the staff and facilities described below, demonstrating the importance of the Core to the overall success of the PPG.
RATIONALE The strongest rationale for creating this core is the need for a high degree of technical skill, experience, and strict quality control in the production and maintenance of transgenic mice. The success of these procedures is directiy linked to the experience of the operator and we have found this structure to be effective in assuring the cost-efficient generation of transgenic mice for similar projects over the 10-year lifespan of this core. The efficiencies inherent in centralizing the maintenance of production stocks and the specialized equipment are also a strong rationale for our proposal. Efficient production, breeding, accurate genotyping and timely culling of unneeded mice will lead to a more efficient use of a very powerful but expensive resource.
|Danhaive, Olivier; Chapin, Cheryl; Horneman, Hart et al. (2015) Surface film formation in vitro by infant and therapeutic surfactants: role of surfactant protein B. Pediatr Res 77:340-6|
|LaFemina, Michael J; Sutherland, Katherine M; Bentley, Trevor et al. (2014) Claudin-18 deficiency results in alveolar barrier dysfunction and impaired alveologenesis in mice. Am J Respir Cell Mol Biol 51:550-8|
|Gonzalez, Robert F; Dobbs, Leland G (2013) Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs. Methods Mol Biol 945:145-59|
|Chapin, Cheryl; Bailey, Nicole A; Gonzales, Linda W et al. (2012) Distribution and surfactant association of carcinoembryonic cell adhesion molecule 6 in human lung. Am J Physiol Lung Cell Mol Physiol 302:L216-25|
|Heine, Vivi M; Griveau, Amelie; Chapin, Cheryl et al. (2011) A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Sci Transl Med 3:105ra104|
|Gonzalez, Robert F; Allen, Lennell; Gonzales, Linda et al. (2010) HTII-280, a biomarker specific to the apical plasma membrane of human lung alveolar type II cells. J Histochem Cytochem 58:891-901|
|Gonzalez, Robert F; Allen, Lennell; Dobbs, Leland G (2009) Rat alveolar type I cells proliferate, express OCT-4, and exhibit phenotypic plasticity in vitro. Am J Physiol Lung Cell Mol Physiol 297:L1045-55|
|Mun, James J; Tam, Connie; Kowbel, David et al. (2009) Clearance of Pseudomonas aeruginosa from a healthy ocular surface involves surfactant protein D and is compromised by bacterial elastase in a murine null-infection model. Infect Immun 77:2392-8|
|Johnson, Meshell; Allen, Lennell; Dobbs, Leland (2009) Characteristics of Cl- uptake in rat alveolar type I cells. Am J Physiol Lung Cell Mol Physiol 297:L816-27|
|Vanderbilt, Jeff N; Allen, Lennell; Gonzalez, Robert F et al. (2008) Directed expression of transgenes to alveolar type I cells in the mouse. Am J Respir Cell Mol Biol 39:253-62|
Showing the most recent 10 out of 172 publications