This PPG was started in September, 1982. The central theme is """"""""the study of the role of vasoactive systems (autocrine, juxtacrine, paracrine and endocrine) in the regulation of renal function and blood pressure (BP) and mediation of end organ damage (EOD)"""""""". The general hypothesis to be tested is that in Ang ll-lnduced Hypertension there is a balance between systems that promote water and sodium retention, hypertension and EOD (Ang II, COX-2 products, ROS and inflammation), and systems that antagonize these effects (Ac- SDKP, activation of the Ang II type 2 receptor, kinins, NO, eicosanoids and the newly discovered cross-talk between the connecting tubule and the afferent arteriole). Alterations of this balance in favor of the former are responsible for retention of water and sodium and development of hypertension and EOD, while alterations of this balance in favor of the latter have therapeutic effects. We will use molecular, physiological, and pharmacological approaches to study vasoactive systems at the subcellular, cellular, and isolated organ levels and in Ang ll-induced hypertension in rabbits, rats and various transgenic mice. In project I we will study whether in Ang II induced hypertension, a novel peptide (Ac-SDKP) alters the balance between systems that promote and oppose EOD in favor of the latter, thus preventing and regressing this process. In project II we will study whether in Ang ll-induced hypertension cardiac damage and dysfunction are antagonized by activation of the AT2 receptor via kinins and NO. In project III we will study whether in Ang ll-induced hypertension, expression of COX-2 and PGE2 production, acting through its EP4 receptor, promotes the development of cardiovascular disease. In project IV, we will study whether in Ang ll-induced hypertension, the renal microcirculation is regulated by a novel mechanism, cross-talk between the connecting tubule and the afferent arteriole, that antagonizes the vasoconstrictor effect of Ang II. In project V, we will study whether in Ang ll-induced hypertension renal function is altered by oxidative stress in the nephron, which causes a decrease in NO and an increase in sodium absorption. Four cores (Administrative, Analytical and Morphological, Mutant Mouse and Biostatistics) will support and facilitate the scientific efforts of the investigators. The PPG provides integration of our efforts, collaboration and sharing of ideas and expertise, thus accelerating acquisition of knowledge on the pathogenesis of hypertension and EOD.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL028982-30
Application #
8120665
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
OH, Youngsuk
Project Start
1982-09-01
Project End
2013-02-14
Budget Start
2011-06-01
Budget End
2013-02-14
Support Year
30
Fiscal Year
2011
Total Cost
$2,447,004
Indirect Cost
Name
Henry Ford Health System
Department
Type
DUNS #
073134603
City
Detroit
State
MI
Country
United States
Zip Code
48202
Cerniello, Flavia M; Carretero, Oscar A; Longo Carbajosa, Nadia A et al. (2017) MAS1 Receptor Trafficking Involves ERK1/2 Activation Through a ?-Arrestin2-Dependent Pathway. Hypertension 70:982-989
Ren, YiLin; Janic, Branislava; Kutskill, Kristopher et al. (2016) Mechanisms of connecting tubule glomerular feedback enhancement by aldosterone. Am J Physiol Renal Physiol 311:F1182-F1188
Ramseyer, Vanesa D; Ortiz, Pablo A; Carretero, Oscar A et al. (2016) Angiotensin II-mediated hypertension impairs nitric oxide-induced NKCC2 inhibition in thick ascending limbs. Am J Physiol Renal Physiol 310:F748-F754
Gu, Xiaosong; Xu, Jiang; Zhu, Liping et al. (2016) Prostaglandin E2 Reduces Cardiac Contractility via EP3 Receptor. Circ Heart Fail 9:
Kumar, Nitin; Nakagawa, Pablo; Janic, Branislava et al. (2016) The anti-inflammatory peptide Ac-SDKP is released from thymosin-?4 by renal meprin-? and prolyl oligopeptidase. Am J Physiol Renal Physiol 310:F1026-34
Zhu, Liping; Yang, Xiao-Ping; Janic, Branislava et al. (2016) Ac-SDKP suppresses TNF-?-induced ICAM-1 expression in endothelial cells via inhibition of I?B kinase and NF-?B activation. Am J Physiol Heart Circ Physiol 310:H1176-83
Saez, Fara; Hong, Nancy J; Garvin, Jeffrey L (2016) Luminal flow induces NADPH oxidase 4 translocation to the nuclei of thick ascending limbs. Physiol Rep 4:
Cerrato, Bruno D; Carretero, Oscar A; Janic, Brana et al. (2016) Heteromerization Between the Bradykinin B2 Receptor and the Angiotensin-(1-7) Mas Receptor: Functional Consequences. Hypertension 68:1039-48
González, Germán E; Rhaleb, N-E; D'Ambrosio, Martin A et al. (2016) Cardiac-deleterious role of galectin-3 in chronic angiotensin II-induced hypertension. Am J Physiol Heart Circ Physiol 311:H1287-H1296
Gonzalez-Vicente, Agustin; Saikumar, Jagannath H; Massey, Katherine J et al. (2016) Angiotensin II stimulates superoxide production by nitric oxide synthase in thick ascending limbs. Physiol Rep 4:

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