The primary purpose of the Analytical and Morphological Core (Core B) is to provide established and centralized assays and expert personnel for projects of the Program Project Grant (PPG), which includes analytical chemistry, enzyme-linked immunoassay (ELISA), radioimmunoassay (RIA), real-time polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA) and histological, morphological and imaging analysis. Analytical chemistry covers measurement of nitrites/nitrates, creatinine, hydroxyproline and kallikrein activity. ELISA will be used to measure N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), prostanoids, cytokines, unnary protein, etc. RIA will be used to measure plasma renin activity and renin and angiotensin concentrations, cyclic adenosine (cAMP) and guanosine monophosphates (cGMP). RT-PCR and EMSA will be performed to measure gene expression and nuclear factor kappaB ( N F K B ) activation and translocation. All chemical analyses and bioassays will be performed on samples obtained from tissues, body fluids or cultured cells. Histomorphology includes tissue processing, embedding, cutting, histological and immunohistochemical staining and photography for measurement of a) infarct size, cardiomyocyte size, capillary density, interstitial and perivascular collagen;2) inflammatory cell infiltration, adhesion molecules, markers of oxidative stress, apoptosis and necrosis in the vasculature, heart and kidney;and c) periodic acid Schiff (PAS) staining for kidney lesions. The Project Leaders will analyze the histological data in a blinded fashion with the assistance of Core B. In addition, the Analytical and Morphological Core will relieve Project Leaders from performing these labor-intensive and time-consuming analyses themselves and instead allow them more time to pursue their scientific efforts. The anticipated Analytical and Morphological Core's effort for each project is estimated as Project I, 55%;Project 11, 30%, Project HI, 10%;and Project IV, 5%.

Public Health Relevance

By centralizing analytical and morphological studies for Project Leaders, Core B will help integrate participating projects by providing consistent, reliable and objectively analytic and morphological assays, which will promote both quality and productivity of participating projects. Core B will ensure cost efficiency in terms of labor and laboratory facilities/equipment to avoid duplication of efforts by individual Project Leaders.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Henry Ford Health System
United States
Zip Code
Gonzalez-Vicente, Agustin; Saikumar, Jagannath H; Massey, Katherine J et al. (2016) Angiotensin II stimulates superoxide production by nitric oxide synthase in thick ascending limbs. Physiol Rep 4:
González, Germán E; Rhaleb, N-E; D'Ambrosio, Martin A et al. (2016) Cardiac-deleterious role of galectin-3 in chronic angiotensin II-induced hypertension. Am J Physiol Heart Circ Physiol 311:H1287-H1296
Ren, Yilin; Janic, Branislava; Kutskill, Kristopher et al. (2016) Mechanisms of Connecting Tubule Glomerular Feedback Enhancement by Aldosterone. Am J Physiol Renal Physiol :ajprenal.00076.2016
Gu, Xiaosong; Xu, Jiang; Zhu, Liping et al. (2016) Prostaglandin E2 Reduces Cardiac Contractility via EP3 Receptor. Circ Heart Fail 9:
Zhu, Liping; Yang, Xiao-Ping; Janic, Branislava et al. (2016) Ac-SDKP suppresses TNF-α-induced ICAM-1 expression in endothelial cells via inhibition of IκB kinase and NF-κB activation. Am J Physiol Heart Circ Physiol 310:H1176-83
Ramseyer, Vanesa Daniela; Ortiz, Pablo A; Carretero, Oscar A et al. (2016) Angiotensin II-mediated hypertension impairs nitric oxide-induced NKCC2 inhibition in thick ascending limbs. Am J Physiol Renal Physiol :ajprenal.00473.2015
Cerrato, Bruno D; Carretero, Oscar A; Janic, Brana et al. (2016) Heteromerization Between the Bradykinin B2 Receptor and the Angiotensin-(1-7) Mas Receptor: Functional Consequences. Hypertension 68:1039-48
Worou, Morel E; Liao, Tang-Dong; D'Ambrosio, Martin et al. (2015) Renal protective effect of N-acetyl-seryl-aspartyl-lysyl-proline in dahl salt-sensitive rats. Hypertension 66:816-22
Peng, Hongmei; Sarwar, Zeyd; Yang, Xiao-Ping et al. (2015) Profibrotic Role for Interleukin-4 in Cardiac Remodeling and Dysfunction. Hypertension 66:582-9
Monzon, Casandra M; Garvin, Jeffrey L (2015) Nitric oxide decreases the permselectivity of the paracellular pathway in thick ascending limbs. Hypertension 65:1245-50

Showing the most recent 10 out of 376 publications