Abstract

The purpose of the Morphology/Cell Culture Core will be to perform tissue processing, sectioning, histochemical and immunohistochemistry staining as well as prepare primary-culture airway epithelial cells for Program investigators and their associates. The Core will be staffed by experienced research technicians to adequately provide these services and will function within an established Morphology and Immunopathology Core that is fully equipped and currently supported by numerous other NIH/NHLBI programs and a Washington University School of Medicine local grant. Similarly, the cell culture component of this proposal will be incorporated into an established Airway Epithelial Cell Core that is fully equipped and currently supported by a Childrens Discovery Institute grant through Washington University School of Medicine. The Morphology/Cell Culture Core will be an integral and essential component of this Program Project Grant application because each of the individual projects proposes numerous experiments that require the services provided by the Core.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL029594-30
Application #
8378777
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
30
Fiscal Year
2012
Total Cost
$237,311
Indirect Cost
$59,579

  Institution

Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Byers, Derek E; Wu, Kangyun; Dang-Vu, Geoffrey et al. (2018) Triggering Receptor Expressed on Myeloid Cells-2 Expression Tracks With M2-Like Macrophage Activity and Disease Severity in COPD. Chest 153:77-86
Wu, Kangyun; Byers, Derek E; Jin, Xiaohua et al. (2015) TREM-2 promotes macrophage survival and lung disease after respiratory viral infection. J Exp Med 212:681-97
Gharib, Sina A; Edelman, Jeffery D; Ge, Lingyin et al. (2015) Acute cellular rejection elicits distinct microRNA signatures in airway epithelium of lung transplant patients. Transplant Direct 1:
Pan, Jie-Hong; Adair-Kirk, Tracy L; Patel, Anand C et al. (2014) Myb permits multilineage airway epithelial cell differentiation. Stem Cells 32:3245-56
Rohani, Maryam G; Pilcher, Brian K; Chen, Peter et al. (2014) Cdc42 inhibits ERK-mediated collagenase-1 (MMP-1) expression in collagen-activated human keratinocytes. J Invest Dermatol 134:1230-1237
Holtzman, Michael J; Byers, Derek E; Alexander-Brett, Jennifer et al. (2014) The role of airway epithelial cells and innate immune cells in chronic respiratory disease. Nat Rev Immunol 14:686-98
Holtzman, Michael J; Byers, Derek E; Brett, Jennifer-Alexander et al. (2014) Linking acute infection to chronic lung disease. The role of IL-33-expressing epithelial progenitor cells. Ann Am Thorac Soc 11 Suppl 5:S287-91
Gu, Xiaoling; Karp, Philip H; Brody, Steven L et al. (2014) Chemosensory functions for pulmonary neuroendocrine cells. Am J Respir Cell Mol Biol 50:637-46
Byers, Derek E; Alexander-Brett, Jennifer; Patel, Anand C et al. (2013) Long-term IL-33-producing epithelial progenitor cells in chronic obstructive lung disease. J Clin Invest 123:3967-82
Chen, Peter; Edelman, Jeffrey D; Gharib, Sina A (2013) Comparative evaluation of miRNA expression between in vitro and in vivo airway epithelium demonstrates widespread differences. Am J Pathol 183:1405-1410

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