Abstract

Core C is a continuing core for Trangenic and Gene Targeting of Mice. In the next grant cycle Core C will assist Project 1 in constructing endothelial specific knockouts of SCAP and STATS on the LDL receptor null background.Core C will assist Projects 1 arid 2 in constructing HO-1 conditional and endothelial specific, knockout of HO-1 on the apoE null background. Core C will assist Project 3 in construction of a MGP-P64Q mutant mouse through gene targeting and will assist Project 3 in the generation of Abcc6-Wnt-promoterbeta- gal reporter double transgenic mice. Core C will provide consultation on breeding and colony management of transgenic mouse models to Project 4. Core C will assist Project 5 construction of, transgenci mice for testing atherosclerosis-related candidate genes. Core C will assist Project 6 with the construction of transgenic mice expressing dominant negatie NR4A receptor in macrophages. Core C will , also provide assistance to Core A in the characterization of primary cultues of mouse aortic endothelial cells by performing qPCR and Western blot analysis.These functions of Core C are essential to ensuring the efficient and cost-effective completion of the specific aims of all of the component Projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL030568-30
Application #
8446363
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
30
Fiscal Year
2013
Total Cost
$188,496
Indirect Cost
$66,096

  Institution

Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Jumabay, Medet; Zhumabai, Jiayinaguli; Mansurov, Nurlan et al. (2018) Combined effects of bone morphogenetic protein 10 and crossveinless-2 on cardiomyocyte differentiation in mouse adipocyte-derived stem cells. J Cell Physiol 233:1812-1822
Mangul, Serghei; Yang, Harry Taegyun; Strauli, Nicolas et al. (2018) ROP: dumpster diving in RNA-sequencing to find the source of 1 trillion reads across diverse adult human tissues. Genome Biol 19:36
Mack, Julia J; Iruela-Arispe, M Luisa (2018) NOTCH regulation of the endothelial cell phenotype. Curr Opin Hematol 25:212-218
Beceiro, Susana; Pap, Attila; Czimmerer, Zsolt et al. (2018) LXR nuclear receptors are transcriptional regulators of dendritic cell chemotaxis. Mol Cell Biol :
Sallam, Tamer; Jones, Marius; Thomas, Brandon J et al. (2018) Transcriptional regulation of macrophage cholesterol efflux and atherogenesis by a long noncoding RNA. Nat Med 24:304-312
Skye, Sarah M; Zhu, Weifei; Romano, Kymberleigh A et al. (2018) Microbial Transplantation With Human Gut Commensals Containing CutC Is Sufficient to Transmit Enhanced Platelet Reactivity and Thrombosis Potential. Circ Res 123:1164-1176
Lin, Liang-Yu; Chun Chang, Sunny; O'Hearn, Jim et al. (2018) Systems Genetics Approach to Biomarker Discovery: GPNMB and Heart Failure in Mice and Humans. G3 (Bethesda) 8:3499-3506
Rahmani, Elior; Schweiger, Regev; Shenhav, Liat et al. (2018) BayesCCE: a Bayesian framework for estimating cell-type composition from DNA methylation without the need for methylation reference. Genome Biol 19:141
Chattopadhyay, Arnab; Yang, Xinying; Mukherjee, Pallavi et al. (2018) Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. Sci Rep 8:9032
Hui, Simon T; Kurt, Zeyneb; Tuominen, Iina et al. (2018) The Genetic Architecture of Diet-Induced Hepatic Fibrosis in Mice. Hepatology 68:2182-2196

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