The function of the BIOCHEMISTRY CORE is to provide instrumentation, facilities and technical expertise for performing routine, state-of-the-art assays of the many lipid species that are of interest to each of the program projects. The success of all three individual projects is dependent on the ready availability of high quality analytical assays, using standardized protocols, for the quantitative determination of the individual lipid species that are central to understanding the biochemical and biological processes under investigation. Performance of these biochemical assays in a centralized facility, rather than in each individual laboratory, is an efficient and economical means for maintaining and using state-of-the-art expertise and instruments for lipid analysis. As necessary to address the specific aims of the individual projects, an additional function of the Core is to develop and implement new analytical methods, generally employing mass spectrometric detection, to measure lipid species that are either novel or difficult to assay by existing approaches. The Core, having recently added a workstation and software for more efficient data processing and multivariate statistical analysis, also is a resource to assist the individual projects with sophisticated processing, evaluation and interpretation of complex data.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL034303-28
Application #
8426127
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
28
Fiscal Year
2013
Total Cost
$275,495
Indirect Cost
$66,514
Name
National Jewish Health
Department
Type
DUNS #
076443019
City
Denver
State
CO
Country
United States
Zip Code
80206
Yun, Bogeon; Lee, HeeJung; Ghosh, Moumita et al. (2014) Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation. J Biol Chem 289:1491-504
Zemski Berry, Karin A; Gordon, William C; Murphy, Robert C et al. (2014) Spatial organization of lipids in the human retina and optic nerve by MALDI imaging mass spectrometry. J Lipid Res 55:504-15
Redente, Elizabeth F; Keith, Rebecca C; Janssen, William et al. (2014) Tumor necrosis factor-* accelerates the resolution of established pulmonary fibrosis in mice by targeting profibrotic lung macrophages. Am J Respir Cell Mol Biol 50:825-37
Caceres, Silvia M; Malcolm, Kenneth C; Taylor-Cousar, Jennifer L et al. (2014) Enhanced in vitro formation and antibiotic resistance of nonattached Pseudomonas aeruginosa aggregates through incorporation of neutrophil products. Antimicrob Agents Chemother 58:6851-60
Henson, Peter M; Bratton, Donna L (2013) Antiinflammatory effects of apoptotic cells. J Clin Invest 123:2773-4
Aldrovandi, Maceler; Hammond, Victoria J; Podmore, Helen et al. (2013) Human platelets generate phospholipid-esterified prostaglandins via cyclooxygenase-1 that are inhibited by low dose aspirin supplementation. J Lipid Res 54:3085-97
Suram, Saritha; Silveira, Lori J; Mahaffey, Spencer et al. (2013) Cytosolic phospholipase A(2)? and eicosanoids regulate expression of genes in macrophages involved in host defense and inflammation. PLoS One 8:e69002
Fernandez-Boyanapalli, Ruby; Goleva, Elena; Kolakowski, Christena et al. (2013) Obesity impairs apoptotic cell clearance in asthma. J Allergy Clin Immunol 131:1041-7, 1047.e1-3
Talahalli, Ramaprasad; Zarini, Simona; Tang, Jie et al. (2013) Leukocytes regulate retinal capillary degeneration in the diabetic mouse via generation of leukotrienes. J Leukoc Biol 93:135-43
Frasch, S Courtney; Fernandez-Boyanapalli, Ruby F; Berry, Karin A Zemski et al. (2013) Neutrophils regulate tissue Neutrophilia in inflammation via the oxidant-modified lipid lysophosphatidylserine. J Biol Chem 288:4583-93

Showing the most recent 10 out of 247 publications