This core facility will provide service in DNA cloning, site-directed mutagenesis, overexpression of recombinant apolipoproteins and mutants in E. Coli, and production of gene constructs to produce transgenic mice. Project 1 requires a large number of site-directed mutants of apo-A-I for structural and functional analyses. Project 8 requires construction and overexpression of partial or complete domains of apoB in E. Coli for biophysical studies. Project 10 requires construction of genes to overexpress peptides and apolipoproteins in mice.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL034343-19
Application #
7083531
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
19
Fiscal Year
2005
Total Cost
$283,687
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
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