The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.
| Huang, Chunfa; Miller, Richard Tyler (2010) Novel Ca receptor signaling pathways for control of renal ion transport. Curr Opin Nephrol Hypertens 19:106-12 |
| Yu, Changqing; Yang, Zhiwei; Ren, Hongmei et al. (2009) D3 dopamine receptor regulation of ETB receptors in renal proximal tubule cells from WKY and SHRs. Am J Hypertens 22:877-83 |
| Zeng, Chunyu; Asico, Laureano D; Yu, Changqing et al. (2008) Renal D3 dopamine receptor stimulation induces natriuresis by endothelin B receptor interactions. Kidney Int 74:750-9 |
| Zeng, Chunyu; Armando, Ines; Luo, Yingjin et al. (2008) Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice. Am J Physiol Heart Circ Physiol 294:H551-69 |
| Zeng, Chunyu; Villar, Van Anthony M; Eisner, Gilbert M et al. (2008) G protein-coupled receptor kinase 4: role in blood pressure regulation. Hypertension 51:1449-55 |
| Resnick, Andrew; Hopfer, Ulrich (2008) Mechanical stimulation of primary cilia. Front Biosci 13:1665-80 |
| Woost, Philip G; Kolb, Robert J; Chang, Chung-Ho et al. (2007) Development of an AT2-deficient proximal tubule cell line for transport studies. In Vitro Cell Dev Biol Anim 43:352-60 |
| Resnick, Andrew; Hopfer, Ulrich (2007) Force-response considerations in ciliary mechanosensation. Biophys J 93:1380-90 |
| Huang, Chunfa; Miller, R Tyler (2007) Regulation of renal ion transport by the calcium-sensing receptor: an update. Curr Opin Nephrol Hypertens 16:437-43 |
| Ulmasov, Barbara; Bruno, Jonathan; Woost, Philip G et al. (2007) Tissue and subcellular distribution of CLIC1. BMC Cell Biol 8:8 |
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