The Shared Instrumentation Core has been supported by the PPG from its inception and currently includes the following laboratories: * Molecular Biology - DNA sequencing, real-time PCR, bead array analysis * Protein Chemistry - Synthesis, purification, analysis, and post-synthesis modification of peptides, HPLC purification of proteins * Flow Cytometry - Fluorescence-based cell sorting and analysis * Biophysics - biomolecular interaction analysis (Biacore) * Hybridoma - production of murine and hamster monoclonal antibodies * Histology - tissue preparation, sectioning and staining * Viral Vector - preparation of virus-based gene delivery and siRNA gene silencing vectors * Confocal Microscope - confocal and multiphoton microscopy * Thrombosis - In vitro and in vivo systems for studying cells under relevant flow conditions In addition to funds received from the PPG. the operation of these core facilities is subsidized with internal financial support received from the Blood Center Research Foundation, through collaborative arrangements with the Medical College of Wisconsin and through user fees assessed for services provided to non-PPG research projects. Most of the equipment within the core laboratories has been purchased with funds made available by the Blood Center Research Foundation and through Shared Instrumentation Grants obtained from the NIH. There have been several additions and upgrades to the major equipment in the BRI Core Labs since the last submission of the PPG. The table below will outline these equipment changes. More detailed descriptions of the equipment available in each Core Laboratory will be presented in the Resources section.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL044612-23
Application #
8434896
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-12-01
Budget End
2013-11-30
Support Year
23
Fiscal Year
2013
Total Cost
$253,820
Indirect Cost
$101,375
Name
Bloodcenter of Wisconsin, Inc.
Department
Type
DUNS #
057163172
City
Milwaukee
State
WI
Country
United States
Zip Code
53233
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Shi, Qizhen; Kuether, Erin L; Chen, Yingyu et al. (2014) Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells. Blood 123:395-403
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Brott, David A; Katein, Anne; Thomas, Heath et al. (2014) Evaluation of von Willebrand factor and von Willebrand factor propeptide in models of vascular endothelial cell activation, perturbation, and/or injury. Toxicol Pathol 42:672-83
Zheng, Yongwei; Yu, Mei; Podd, Andrew et al. (2013) Critical role for mouse marginal zone B cells in PF4/heparin antibody production. Blood 121:3484-92
Qi, Xiaopeng; Hong, Jessie; Chaves, Lee et al. (2013) Antagonistic regulation by the transcription factors C/EBP* and MITF specifies basophil and mast cell fates. Immunity 39:97-110
Tourdot, Benjamin E; Brenner, Michelle K; Keough, Kathleen C et al. (2013) Immunoreceptor tyrosine-based inhibitory motif (ITIM)-mediated inhibitory signaling is regulated by sequential phosphorylation mediated by distinct nonreceptor tyrosine kinases: a case study involving PECAM-1. Biochemistry 52:2597-608

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