Abstract

Purpose This core will provide chemical synthesis services and expertise in probe development for subprojects within the Program. The majority of the service component will be developed to synthesis, purification, and analysis of peptides and caged compounds. Technical assistance in the use of commercial caged compounds will be provided including chromatographic analysis of compound purity, and determination of extent of photo-conversion under specific experimental conditions. Many of the compounds being used in the subprojects are not commercially available and mus be prepared on a regular basis. In some instances, compounds need to be radiolabeled, or have biotin or fluorophores attached for addressing specific issues regarding a compounds stability, localization, or effective concentration in cells. Labeling and the flexibility to otherwise customize reagents for specific purposes represent two of many significant advantages of having 'in house' chemical synthesis capabilities. Core staff in consultation with subproject personnel will design and implement synthetic strategies based upon the requirements of the physiological or biochemical system under investigation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL047053-08
Application #
6600929
Study Section
Project Start
2002-06-01
Project End
2003-05-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
$199,591
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Druckenbrod, Noah R; Powers, Patricia A; Bartley, Christopher R et al. (2008) Targeting of endothelin receptor-B to the neural crest. Genesis 46:396-400
Brickson, S; Fitzsimons, D P; Pereira, L et al. (2007) In vivo left ventricular functional capacity is compromised in cMyBP-C null mice. Am J Physiol Heart Circ Physiol 292:H1747-54
Vatta, Matteo; Ackerman, Michael J; Ye, Bin et al. (2006) Mutant caveolin-3 induces persistent late sodium current and is associated with long-QT syndrome. Circulation 114:2104-12
Stelzer, Julian E; Larsson, Lars; Fitzsimons, Daniel P et al. (2006) Activation dependence of stretch activation in mouse skinned myocardium: implications for ventricular function. J Gen Physiol 127:95-107
Stelzer, Julian E; Fitzsimons, Daniel P; Moss, Richard L (2006) Ablation of myosin-binding protein-C accelerates force development in mouse myocardium. Biophys J 90:4119-27
Singla, Dinender K; Hacker, Timothy A; Ma, Lining et al. (2006) Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol 40:195-200
Muthukumarana, Poorni A D S; Lyons, Gary E; Miura, Yuji et al. (2006) Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance. Int Immunopharmacol 6:1993-2001
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Acceleration of stretch activation in murine myocardium due to phosphorylation of myosin regulatory light chain. J Gen Physiol 128:261-72
Stelzer, Julian E; Patel, Jitandrakumar R; Moss, Richard L (2006) Protein kinase A-mediated acceleration of the stretch activation response in murine skinned myocardium is eliminated by ablation of cMyBP-C. Circ Res 99:884-90
Balijepalli, Ravi C; Foell, Jason D; Hall, Duane D et al. (2006) Localization of cardiac L-type Ca(2+) channels to a caveolar macromolecular signaling complex is required for beta(2)-adrenergic regulation. Proc Natl Acad Sci U S A 103:7500-5

Showing the most recent 10 out of 103 publications