The Vector Core at the University of lowa is integrated into multiple gene therapy projects directed at the study of diseases of the lung, eye, brain, liver, heart, muscle, and the cardiovascular and neuromuscular systems. Vector Core staff are also active participants in the development of gene transfer technologies in the Cardiovascular, Macular Degeneraflon, and Cystic Fibrosis Centers. The interaction with multiple investigators from various disciplines allows for cross- fertilizaflon of ideas, technical advancements, and innovations in vector design. The overall objective of the Vector Core facility is to support investigators in the use of gene transfer technologies. This includes consultation with investigators, development of novel vectors, collaborative testing of vectors generated for function and purity, and flnally rouflne preparaflon. The Vector Core staff and investigators are in close contact through all phases of vector design and generation. As a part of the service the Vector Core will provide purifled and concentrated preparations of recombinant adenovirus, adeno-associated virus (AAV), and lentivirus. This facility will also provide access to standard cell lines, expression plasmids, and stocks of recombinant reporter viruses. Thus, the Vector Core serves as both a research and development facility for gene transfer studies, and a service facility for routine vector preparations. The Vector Core is committed to producing 'state of the art'gene transfer reagents. This facility addresses this commitment via multiple avenues. First, the Vector Core staff keeps abreast of many different gene transfer technologies from the current literature and scientiflc meetings. Second, the Vector Core provides service to a broad scope of users across institutions and countries. This fosters inter-collegiate communication with focused efforts at developing improved vectors and delivery methods. Finally, a continuum of new ideas from our close collaboraflons with Drs. McCray, Zabner and Welsh, insures that the lowa Gene Transfer community has access to advancements in the 'state of the art'vector.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL051670-19
Application #
8382315
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
19
Fiscal Year
2012
Total Cost
$283,562
Indirect Cost
$91,028
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Pillay, Sirika; Zou, Wei; Cheng, Fang et al. (2017) AAV serotypes have distinctive interactions with domains of the cellular receptor AAVR. J Virol :
Meyerholz, David K; Reznikov, Leah R (2017) Simple and reproducible approaches for the collection of select porcine ganglia. J Neurosci Methods 289:93-98
Sinn, P L; Hwang, B-Y; Li, N et al. (2017) Novel GP64 envelope variants for improved delivery to human airway epithelial cells. Gene Ther 24:674-679
Paemka, Lily; McCullagh, Brian N; Abou Alaiwa, Mahmoud H et al. (2017) Monocyte derived macrophages from CF pigs exhibit increased inflammatory responses at birth. J Cyst Fibros 16:471-474
Meyerholz, David K; Ofori-Amanfo, Georgina K; Leidinger, Mariah R et al. (2017) Immunohistochemical Markers for Prospective Studies in Neurofibromatosis-1 Porcine Models. J Histochem Cytochem 65:607-618
Li, Xiaopeng; Vargas Buonfiglio, Luis G; Adam, Ryan J et al. (2017) Cystic Fibrosis Transmembrane Conductance Regulator Potentiation as a Therapeutic Strategy for Pulmonary Edema: A Proof-of-Concept Study in Pigs. Crit Care Med 45:e1240-e1246
Ramsey, Bonnie W; Welsh, Michael J (2017) AJRCCM: 100-Year Anniversary. Progress along the Pathway of Discovery Leading to Treatment and Cure of Cystic Fibrosis. Am J Respir Crit Care Med 195:1092-1099
Cook, Daniel P; Adam, Ryan J; Zarei, Keyan et al. (2017) CF airway smooth muscle transcriptome reveals a role for PYK2. JCI Insight 2:
Hisert, Katherine B; Heltshe, Sonya L; Pope, Christopher et al. (2017) Restoring Cystic Fibrosis Transmembrane Conductance Regulator Function Reduces Airway Bacteria and Inflammation in People with Cystic Fibrosis and Chronic Lung Infections. Am J Respir Crit Care Med 195:1617-1628
Cooney, Ashley L; Abou Alaiwa, Mahmoud H; Shah, Viral S et al. (2016) Lentiviral-mediated phenotypic correction of cystic fibrosis pigs. JCI Insight 1:

Showing the most recent 10 out of 161 publications