The purpose of the Analytical and Phenotyping Core is to provide state-of-the-art cardiovascular and biochemical analysis of sympathetic baroreflex function, including measurements of sympathetic nerve traffic, catechols, and norepinephrine clearance and spillover. This resource has been critical to the recognition and characterization by our group of new disorders involving the autonomic nervous system, such as dopamine-beta-hydroxylase deficiency. A variety of techniques have been established in this Core to support individual projects, including the determination of plasma volume (Project land 4), heart rate and blood pressure variability (Project 1,2,3, and 4), and baroreflex function (Project 1 and 4). Especially Project 2 among all the others will utilize biochemical evaluation of sympathetic activity which complements other methods of autonomic evaluation detailed in the Clinical Core. Expertise in analysis of ultradian rhythms of cardiovascular parameters for phenotyping will be utilized in Project 1, 3, and 4. Project 1 and 4 will use our novel signal processing tools for neurological signals. In addition, we have implemented an animal phenotyping facility, adapting the methods developed to evaluate autonomic function in humans, to the mice which will be used mainly in Project 1. This includes, among other things, monitoring intra-arterial blood pressure in awake unrestricted mice, monitoring renal sympathetic nerve activity, baroreflex testing, osmopressor tests, and high fidelity ECG, in intact and sinoaortic- denervated mice. Genetically-modified mice are increasingly important in translating autonomic knowledge, and this phenotyping mouse laboratory will be essential in this regard. In this funding period, the Core will provide similar biochemical analytical and phenotyping services but with focus on human data including enhanced signal processing, data reduction with automatic database feeds, links between existing databases, development of data exploration tools for all projects.

Public Health Relevance

The Analytical and Phenotyping Core provides methods to determine how well autonomic control is functioning in humans and in mice. It includes analysis of biological samples, assessment of performance of heart and vessels, and central and peripheral autonomic control. Mice with a genetic defect will be used to study a disease of blood pressure control in a way it is not possible to do in humans.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL056693-18
Application #
8661207
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
18
Fiscal Year
2014
Total Cost
$248,666
Indirect Cost
$90,298
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Biaggioni, Italo; Calhoun, David A (2016) Sympathetic Activity, Hypertension, and The Importance of a Good Night's Sleep. Hypertension 68:1338-1339
Harder, René; Malow, Beth A; Goodpaster, R Lucas et al. (2016) Heart rate variability during sleep in children with autism spectrum disorder. Clin Auton Res 26:423-432
Mar, Philip L; Nwazue, Victor; Black, Bonnie K et al. (2016) Valsalva Maneuver in Pulmonary Arterial Hypertension: Susceptibility to Syncope and Autonomic Dysfunction. Chest 149:1252-60
Shibao, Cyndya A; Celedonio, Jorge E; Ramirez, Claudia E et al. (2016) A Common CD36 Variant Influences Endothelial Function and Response to Treatment with Phosphodiesterase 5 Inhibition. J Clin Endocrinol Metab 101:2751-8
Adefurin, Abiodun; Darghosian, Leon; Okafor, Chimalum et al. (2016) Alpha2A adrenergic receptor genetic variation contributes to hyperglycemia after myocardial infarction. Int J Cardiol 215:482-6
Raj, Satish R (2016) Row, row, row your way to treating postural tachycardia syndrome. Heart Rhythm 13:951-2
Joy, Nino G; Perkins, Jennifer M; Mikeladze, Maia et al. (2016) Comparative effects of acute hypoglycemia and hyperglycemia on pro-atherothrombotic biomarkers and endothelial function in non-diabetic humans. J Diabetes Complications 30:1275-81
Lateef, Dalya M; Xiao, Cuiying; Brychta, Robert J et al. (2016) Bombesin-like receptor 3 regulates blood pressure and heart rate via a central sympathetic mechanism. Am J Physiol Heart Circ Physiol 310:H891-8
Harder, Rene; Diedrich, Andre; Whitfield, Jonathan S et al. (2016) Smart Multi-Frequency Bioelectrical Impedance Spectrometer for BIA and BIVA Applications. IEEE Trans Biomed Circuits Syst 10:912-9
Iwuchukwu, Otito F; Ramirez, Andrea H; Shi, Yaping et al. (2016) Genetic determinants of variability in warfarin response after the dose-titration phase. Pharmacogenet Genomics 26:510-516

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