Core A: Mouse Coagulation Laboratory The Mouse Coagulation Laboratory Core A has the goal to serve every project in the Program. It will provide an array of assays assessing coagulation, and anticoagulant activities, as well as plasma lipid profiles. The Core will also provide assays of inflammatory cytokines and of adipokines, using a mutiplex format with the Luminex Technology. Core A will also use established kits for mouse proteins associated with thrombosis, and atherosclerosis. The specific assays that are available for each of the projects are indicated below. Core A will also coordinate the high through-put screening of small molecules by the Center for Chemical Genomics. Project 1: Core A will perform standard clotting analysis of murine plasma, assays will include FV activity and antigen, APC activity, TFPI activity, D-Dimer, thrombin-antithrombin complex and prothrombin fragment 1+2 assays. Assays of Adamts13 activity will also be performed. Project 2: Core A will evaluate the effect of FVIII expression on ER stress in the liver. Assays will include FVIII activity and antigen, and analysis of inflammatory responses as well as adipokine/cytokine profiles, and metabolic parameters such as lipid profiles. Project 2 will use the CCG component of Core A to screen for small molecule inhibitors of CHOP using unique cell-based assays developed by the Kaufman Laboratory. Project 3: Core A will assist the investigators of Project 3 by measuring plasma cytokine levels in studies of the murine response to Group A Streptococcal infection using a mutiplex format with the Luminex Technology. Project 3 will also use the CCG component of Core A to screen for small molecule inhibitors of Group A Streptococcus virulence factors such as streptokinase. The overall goals of Core A are to enhance the success of each individual project by providing rigorous, standardized assays and to ensure that the combined Program is stronger than each of its individual parts.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL057346-14
Application #
8377023
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
14
Fiscal Year
2012
Total Cost
$186,461
Indirect Cost
$57,625
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Ji, Yan; Weng, Zhen; Fish, Philip et al. (2016) Pharmacological Targeting of Plasminogen Activator Inhibitor-1 Decreases Vascular Smooth Muscle Cell Migration and Neointima Formation. Arterioscler Thromb Vasc Biol 36:2167-2175
Khoriaty, Rami; Everett, Lesley; Chase, Jennifer et al. (2016) Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep 6:27802
Xu, Xianjin; Ma, Zhiwei; Sun, Hongmin et al. (2016) SM-TF: A structural database of small molecule-transcription factor complexes. J Comput Chem 37:1559-64
Emmer, Brian T; Ginsburg, David; Desch, Karl C (2016) Von Willebrand Factor and ADAMTS13: Too Much or Too Little of a Good Thing? Arterioscler Thromb Vasc Biol 36:2281-2282
Khoobchandani, Menka; Katti, Kavita; Maxwell, Adam et al. (2016) Laminin Receptor-Avid Nanotherapeutic EGCg-AuNPs as a Potential Alternative Therapeutic Approach to Prevent Restenosis. Int J Mol Sci 17:316
Tomberg, Kärt; Khoriaty, Rami; Westrick, Randal J et al. (2016) Spontaneous 8bp Deletion in Nbeal2 Recapitulates the Gray Platelet Syndrome in Mice. PLoS One 11:e0150852
Fribley, Andrew M; Miller, Justin R; Brownell, Amy L et al. (2015) Celastrol induces unfolded protein response-dependent cell death in head and neck cancer. Exp Cell Res 330:412-22
Wu, Jianbo; Strawn, Tammy L; Luo, Mao et al. (2015) Plasminogen activator inhibitor-1 inhibits angiogenic signaling by uncoupling vascular endothelial growth factor receptor-2-αVβ3 integrin cross talk. Arterioscler Thromb Vasc Biol 35:111-20
Sun, Hongmin (2015) Factor V: an active player in inflammation. Blood 126:2352-3
Kretz, Colin A; Dai, Manhong; Soylemez, Onuralp et al. (2015) Massively parallel enzyme kinetics reveals the substrate recognition landscape of the metalloprotease ADAMTS13. Proc Natl Acad Sci U S A 112:9328-33

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