Core B: Genetics Core The Genetics Core continues to build on the highly-successful service functions provided to PPG researchers in the previous grant. With highly-trained personnel and specialized instrumentation, this Core provides our researchers with transgenic mouse production, knock-out mouse production, high quality DMA sequencing and microsatellite genotyping. These facilities were well-known among national Core labs for their outstanding technical competence and cost-effectiveness. The transgenic animal lab, under the management of Dr. Thomas Saunders, has accumulated 18 years of experience producing transgenic mice, and 15 years experience in genetic knock-out methodology. The DMA sequencing lab has been in operation for 14 years, the last 12 of which were under the direction of Dr. Lyons. That laboratory has an outstanding reputation for reliability and cost-effectiveness. They recently acquired an Illumina BeadStation 500GX, and have begun providing high-throughput SNP typing services. All four of the PPG projects depend heavily on the Genetics Core. The services described here will be provided on a recharge basis, with members of this Program Project receiving preferential discounts.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL057346-15
Application #
8450258
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
15
Fiscal Year
2013
Total Cost
$132,350
Indirect Cost
$54,858
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Cao, Stewart Siyan; Kaufman, Randal J (2014) Endoplasmic reticulum stress and oxidative stress in cell fate decision and human disease. Antioxid Redox Signal 21:396-413
Adams, Elizabeth J; Chen, Xiao-Wei; O'Shea, K Sue et al. (2014) Mammalian COPII coat component SEC24C is required for embryonic development in mice. J Biol Chem 289:20858-70
Everett, Lesley A; Cleuren, Audrey C A; Khoriaty, Rami N et al. (2014) Murine coagulation factor VIII is synthesized in endothelial cells. Blood 123:3697-705
Ferris, Sean P; Kodali, Vamsi K; Kaufman, Randal J (2014) Glycoprotein folding and quality-control mechanisms in protein-folding diseases. Dis Model Mech 7:331-41
Han, Jaeseok; Kaufman, Randal J (2014) Measurement of the unfolded protein response to investigate its role in adipogenesis and obesity. Methods Enzymol 538:135-50
Groenendyk, Jody; Peng, Zhenling; Dudek, Elzbieta et al. (2014) Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54
Wang, Shiyu; Kaufman, Randal J (2014) How does protein misfolding in the endoplasmic reticulum affect lipid metabolism in the liver? Curr Opin Lipidol 25:125-32
Ji, Y; Fish, P M; Strawn, T L et al. (2014) C-reactive protein induces expression of tissue factor and plasminogen activator inhibitor-1 and promotes fibrin accumulation in vein grafts. J Thromb Haemost 12:1667-77
Cao, Stewart S; Wang, Miao; Harrington, Jane C et al. (2014) Phosphorylation of eIF2? is dispensable for differentiation but required at a posttranscriptional level for paneth cell function and intestinal homeostasis in mice. Inflamm Bowel Dis 20:712-22
Khoriaty, Rami; Vasievich, Matthew P; Jones, Morgan et al. (2014) Absence of a red blood cell phenotype in mice with hematopoietic deficiency of SEC23B. Mol Cell Biol 34:3721-34

Showing the most recent 10 out of 143 publications