Core D: Morphology Core The purpose of the Core D (Morphology Core) is to provide state-of-the-art, standard and specialized morphology and histology services to the various projects of this Program Project grant effort. The Core D (Morphology Core) Director: David Gordon, and the Co-director: He Wang will continually meet with the various investigators of our projects to discuss experimental design and to mutually formulate the gross, microscopic and histologic studies to be performed. Standard procedures shall include assistance with tissue harvesting and fixation, further tissue processing/embedding, immunocytochemistry and in situ mRNA hybridization. Specialized procedures shall include performing autopsies on selected new genetically engineered mice to look for: congenital anomalies, evidence of thrombosis and/or hemorrhage, and other pathology which may be characteristic of the specific genetic alteration. We will also assist individual projects in working out the conditions for using specific new immunostaining and in situ hybridization methods (e.g. for newly acquired antibodies or probes), with the appropriate controls, and with other morphology techniques as the need arises (e.g.laser capture microdissection). Finally this Morphology Core will assist investigators in documenting the findings via routine photomicroscopy and selected morphometry.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL057346-15
Application #
8450262
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
15
Fiscal Year
2013
Total Cost
$132,478
Indirect Cost
$54,857
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Cao, Stewart Siyan; Kaufman, Randal J (2014) Endoplasmic reticulum stress and oxidative stress in cell fate decision and human disease. Antioxid Redox Signal 21:396-413
Adams, Elizabeth J; Chen, Xiao-Wei; O'Shea, K Sue et al. (2014) Mammalian COPII coat component SEC24C is required for embryonic development in mice. J Biol Chem 289:20858-70
Everett, Lesley A; Cleuren, Audrey C A; Khoriaty, Rami N et al. (2014) Murine coagulation factor VIII is synthesized in endothelial cells. Blood 123:3697-705
Ferris, Sean P; Kodali, Vamsi K; Kaufman, Randal J (2014) Glycoprotein folding and quality-control mechanisms in protein-folding diseases. Dis Model Mech 7:331-41
Han, Jaeseok; Kaufman, Randal J (2014) Measurement of the unfolded protein response to investigate its role in adipogenesis and obesity. Methods Enzymol 538:135-50
Groenendyk, Jody; Peng, Zhenling; Dudek, Elzbieta et al. (2014) Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54
Wang, Shiyu; Kaufman, Randal J (2014) How does protein misfolding in the endoplasmic reticulum affect lipid metabolism in the liver? Curr Opin Lipidol 25:125-32
Ji, Y; Fish, P M; Strawn, T L et al. (2014) C-reactive protein induces expression of tissue factor and plasminogen activator inhibitor-1 and promotes fibrin accumulation in vein grafts. J Thromb Haemost 12:1667-77
Cao, Stewart S; Wang, Miao; Harrington, Jane C et al. (2014) Phosphorylation of eIF2? is dispensable for differentiation but required at a posttranscriptional level for paneth cell function and intestinal homeostasis in mice. Inflamm Bowel Dis 20:712-22
Khoriaty, Rami; Vasievich, Matthew P; Jones, Morgan et al. (2014) Absence of a red blood cell phenotype in mice with hematopoietic deficiency of SEC23B. Mol Cell Biol 34:3721-34

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