The purpose of Core A is to provide state-of-the-art measurements of reactive oxygen species (ROS) and nitric oxide (NO) in cells, tissues and membranes for participants of the PPG. CORE A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy and High Pressure Liquid Chromatography (HPLC). This Core facility has existed since 1999 and has supported studies of superoxide (O2~) and NO in vascular cells in many publications from Drs. Harrison, Griendling, Dudley and Jo (1-10). It has recently validated HPLC detection of O2* in intact tissues by monitoring formation of 2-hydroxyethidium from dihydroethidium (11). This facility will significantly enhance the work of the individual laboratories and will enhance collaborations between participants of the PPG.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Vanderbilt University Medical Center
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Trott, Daniel W; Thabet, Salim R; Kirabo, Annet et al. (2014) Oligoclonal CD8+ T cells play a critical role in the development of hypertension. Hypertension 64:1108-15
Gao, Ge; Brahmanandam, Vikram; Raicu, Mihai et al. (2014) Enhanced risk profiling of implanted defibrillator shocks with circulating SCN5A mRNA splicing variants: a pilot trial. J Am Coll Cardiol 63:2261-9
Dikalov, Sergey I; Harrison, David G (2014) Methods for detection of mitochondrial and cellular reactive oxygen species. Antioxid Redox Signal 20:372-82
Jeong, Euy-Myoung; Dudley Jr, Samuel C (2014) New diagnostic and therapeutic possibilities for diastolic heart failure. R I Med J (2013) 97:35-7
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Rutledge, Cody A; Ng, Fu Siong; Sulkin, Matthew S et al. (2014) c-Src kinase inhibition reduces arrhythmia inducibility and connexin43 dysregulation after myocardial infarction. J Am Coll Cardiol 63:928-34
Wu, Jing; Thabet, Salim R; Kirabo, Annet et al. (2014) Inflammation and mechanical stretch promote aortic stiffening in hypertension through activation of p38 mitogen-activated protein kinase. Circ Res 114:616-25
Weyand, Cornelia M; Yang, Zhen; Goronzy, Jorg J (2014) T-cell aging in rheumatoid arthritis. Curr Opin Rheumatol 26:93-100
Qi, Qian; Liu, Yi; Cheng, Yong et al. (2014) Diversity and clonal selection in the human T-cell repertoire. Proc Natl Acad Sci U S A 111:13139-44

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