The Cell Morphology Core is an independent and fully equipped laboratory capable of conducting a wide array of studies necessary to characterize tissue and cell morphology, as well as localize transgenes and transgene products. This Core is directed by Dr. Peter Bell and is housed in 800 square feet of dedicated space and includes two fluorescent microscopes, one electron microscope, and all the equipment necessary to process tissues and cells for a wide array of histological analysis. The Core will provide extensive support to all three of the projects in the area of 1) preparation of tissues for histopathologic analysis, 2) special stains for more detailed characterization of pathologic specimens, 3) analysis of tissues for transgene expression using immunqhistochemistry and in situ hybridization;4) quantitative analysis of reporter gene expression in whole liver and liver sections using the Xenogen technology;and 5) EM analysis of vector preps.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL059407-15
Application #
8502520
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
15
Fiscal Year
2013
Total Cost
$100,889
Indirect Cost
$38,988
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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Ibrahim, Salam; Somanathan, Suryanarayan; Billheimer, Jeffrey et al. (2016) Stable liver-specific expression of human IDOL in humanized mice raises plasma cholesterol. Cardiovasc Res 110:23-9
Calcedo, Roberto; Wilson, James M (2016) AAV Natural Infection Induces Broad Cross-Neutralizing Antibody Responses to Multiple AAV Serotypes in Chimpanzees. Hum Gene Ther Clin Dev 27:79-82
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Xie, Jun; Burt, Daniel Robert; Gao, Guangping (2015) Adeno-associated virus-mediated microRNA delivery and therapeutics. Semin Liver Dis 35:81-8
Ahmed, Seemin S; Gao, Guangping (2015) Making the White Matter Matters: Progress in Understanding Canavan's Disease and Therapeutic Interventions Through Eight Decades. JIMD Rep 19:11-22
Wang, Lili; Bell, Peter; Somanathan, Suryanarayan et al. (2015) Comparative Study of Liver Gene Transfer With AAV Vectors Based on Natural and Engineered AAV Capsids. Mol Ther 23:1877-87

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