This Core will be housed at the Cincinnati Children's Hospital Medical Center within the Division of Molecular Cardiovascular Biology. Although spatially separated from members of the Program Project Grant team. Dr. Robbins has been collaborating with them for years and supplies the current Program Project Grant investigators and Cores with both mice and protein fragments. The Core will be critical for the studies proposed in all of the Program Project Grant's Component's as it will be responsible for the design, production, colony maintenance and shipment of ail transgenic mice that are proposed by the Program Project Grant's investigators and Core B. The Core will also produce the different proteins, protein fragments and antibodies needed for the in vitro and isolated systems studies. As such, it forms an integral part of the Program Project Grant's foundation. The Core's responsibilities are: ? Design and build all constructs for bacterial- or Sacu/o-based MyBP-C protein and protein fragment production. ? Grow and purify all protein fragments in sufficient quantities to satisfy the investigators'needs ? Design, build and generate all transgenic mice that are proposed in the Program Project Grant. ? Maintain sufficient numbers of all lines in barrier facilities so that they can be distributed as needed to the investigators as well as to other scientists who request them. ? Serve as a central clearing house for the preparation, maintenance and quality control of the cMyBP-C specific antibodies ? Maintain an adequate inventory of all of the above and arrange for convenient shipping to the individual investigators or Core Leaders.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL059408-15
Application #
8611951
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2016-01-31
Budget Start
2014-02-01
Budget End
2015-01-31
Support Year
15
Fiscal Year
2014
Total Cost
$515,845
Indirect Cost
$3,776
Name
University of Vermont & St Agric College
Department
Type
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Yang, Shixin; Woodhead, John L; Zhao, Fa-Qing et al. (2016) An approach to improve the resolution of helical filaments with a large axial rise and flexible subunits. J Struct Biol 193:45-54
Warshaw, David M (2016) HEART DISEASE. Throttling back the heart's molecular motor. Science 351:556-7
James, Jeanne; Robbins, Jeffrey (2016) Healing a Heart Through Genetic Intervention. Circ Res 118:920-2
Mun, Ji Young; Kensler, Robert W; Harris, Samantha P et al. (2016) The cMyBP-C HCM variant L348P enhances thin filament activation through an increased shift in tropomyosin position. J Mol Cell Cardiol 91:141-7
Previs, Michael J; Mun, Ji Young; Michalek, Arthur J et al. (2016) Phosphorylation and calcium antagonistically tune myosin-binding protein C's structure and function. Proc Natl Acad Sci U S A 113:3239-44
Gupta, Manish K; McLendon, Patrick M; Gulick, James et al. (2016) UBC9-Mediated Sumoylation Favorably Impacts Cardiac Function in Compromised Hearts. Circ Res 118:1894-905
Michalek, Arthur J; Kennedy, Guy G; Warshaw, David M et al. (2015) Flexural Stiffness of Myosin Va Subdomains as Measured from Tethered Particle Motion. J Biophys 2015:465693
LeWinter, Martin M; Palmer, Bradley M (2015) Updating the physiology and pathophysiology of cardiac Myosin-binding protein-C. Circ Heart Fail 8:417-21
McLendon, Patrick M; Robbins, Jeffrey (2015) Proteotoxicity and cardiac dysfunction. Circ Res 116:1863-82
Kirk, Jonathan A; Chakir, Khalid; Lee, Kyoung Hwan et al. (2015) Pacemaker-induced transient asynchrony suppresses heart failure progression. Sci Transl Med 7:319ra207

Showing the most recent 10 out of 162 publications