Core C, the Molecular Resources Core, will provide molecular reagents and services to all four projects and will be crucial to the completion of the specific aims of each proposal in the Program Project. The proposal for a Molecular Resources Core is a continuation of the original Core C. The overall function of Core C is to provide molecular reagents and expertise, including generation of deletion and site-directed mutants and incorporation into the desired expression vectors, generation of adenoviral and retroviral constructs for transducfion of primary endothelial cells, genotyping of knockout mice, designing and generating appropriate siRNA based constructs, sequencing and verification of constructs obtained from other investigators, molecular biology training to project personnel, and overseeing and maintaining instrumentation for efficient transfection of endothelial cells. Core C will also provide expertise for proteomic analysis and oxidant measurements. This core will be directed by Dr. Randal A. Skidgel and molecular reagents and services will be provided by a Senior Research Specialist (Dr. Tiffany Sharma), Research Specialist (Debra Salvi) and Research Assistant Professor (Dr. Fulong Tan). Dr. Neil Kelleher, Professor and Director of the Proteomics Center of Excellence, will advise, oversee and carry out proteomic analysis by mass spectrometry. Dr. Marcelo Bonini, Assistant Professor, will provide expertise, advice and will carry out measurement of peroxynitrite and other oxidants to dissect their roles in regulating lung vascular endothelial barrier barrier function. Consolidating these efforts in Core C will result in increased speed and efficiency in accomplishing the research tasks outlined in each of the projects and uniformity of approaches and methods so that results from each project can be compared. In addition, it will allow strict quality control and consistency of molecular reagents to be used by the various projects. Furthermore, an added and important benefit of the centralized Core C will be to markedly decrease expenses compared to a scenario in which each project would generate its own molecular resources. Regular meetings of the Core Leader with the Senior Research Specialist and project leaders will assure coordination and prioritization of the generation of molecular reagents and assure that the desired reagents are provided in a timely manner to the component projects.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL060678-14
Application #
8620696
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
14
Fiscal Year
2014
Total Cost
$591,267
Indirect Cost
$214,662
Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
Jiang, Chunling; Liu, Zheng; Hu, Rong et al. (2017) Inactivation of Rab11a GTPase in Macrophages Facilitates Phagocytosis of Apoptotic Neutrophils. J Immunol 198:1660-1672
Mittal, Manish; Nepal, Saroj; Tsukasaki, Yoshikazu et al. (2017) Response by Mittal et al to Letter Regarding Article, ""Neutrophil Activation of Endothelial Cell-Expressed TRPM2 Mediates Transendothelial Neutrophil Migration and Vascular Injury"". Circ Res 121:e87
Oliveira, Suellen D S; Castellon, Maricela; Chen, Jiwang et al. (2017) Inflammation-induced caveolin-1 and BMPRII depletion promotes endothelial dysfunction and TGF-?-driven pulmonary vascular remodeling. Am J Physiol Lung Cell Mol Physiol 312:L760-L771
Potje, Simone R; Chen, Zhenlong; Oliveira, Suellen D'Arc S et al. (2017) Nitric oxide donor [Ru(terpy)(bdq)NO]3+ induces uncoupling and phosphorylation of endothelial nitric oxide synthase promoting oxidant production. Free Radic Biol Med 112:587-596
Gong, Haixia; Liu, Menglin; Klomp, Jeff et al. (2017) Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells. Sci Rep 7:42127
Gu, Wei; Yao, Lun; Li, Lexing et al. (2017) ICAM-1 regulates macrophage polarization by suppressing MCP-1 expression via miR-124 upregulation. Oncotarget 8:111882-111901
Tsang, Kit Man; Hyun, James S; Cheng, Kwong Tai et al. (2017) Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?. Stem Cell Reports 9:796-806
Park, Thomas J; Reznick, Jane; Peterson, Bethany L et al. (2017) Fructose-driven glycolysis supports anoxia resistance in the naked mole-rat. Science 356:307-311
Yazbeck, Pascal; Tauseef, Mohammad; Kruse, Kevin et al. (2017) STIM1 Phosphorylation at Y361 Recruits Orai1 to STIM1 Puncta and Induces Ca2+ Entry. Sci Rep 7:42758
Marsboom, Glenn; Chen, Zhenlong; Yuan, Yang et al. (2017) Aberrant caveolin-1-mediated Smad signaling and proliferation identified by analysis of adenine 474 deletion mutation (c.474delA) in patient fibroblasts: a new perspective on the mechanism of pulmonary hypertension. Mol Biol Cell 28:1177-1185

Showing the most recent 10 out of 195 publications