Abstract

The Proteomics Core conducts or consults with the four projects on the seven following experiments. The major techniques involved are 2D-DIGE, mass spectrometry, and OFFGEL fractionation. The major experiments we will provide are: (1) To analyze TnT, Tnl, RLC,TM, MyBP-C, CapZ, and FAK phosphorylation levels using 2D-DIGE. (2) To investigate the sub-proteomic myofibrillar protein expression profile (isoform switch levels along with abundance changes) with 2-D-DIGE. (3) To identify proteins from polyacrylamide gels by in-gel digest or from OFFGEL fractions digested in solution followed by LC-MS/MS analysis on a high mass accuracy/resolution mass analyzer. (4) To identify the phosphorylation sites of proteins of interest by LC-MS/MS. (5) To identify oxidized proteins by Oxyblot, 2D-diagonal gels, mass spectrometry. (6) To identify the cross-linked sites by MALDI-TOF/TOF and LC-MS/MS. (7) To identify alterations and/or abundance of lipids in the myocardium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL062426-15
Application #
8629555
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
15
Fiscal Year
2014
Total Cost
$304,424
Indirect Cost
$100,547

  Institution

Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Dvornikov, Alexey V; de Tombe, Pieter P; Xu, Xiaolei (2018) Phenotyping cardiomyopathy in adult zebrafish. Prog Biophys Mol Biol 138:116-125
Le, Long V; Mohindra, Priya; Fang, Qizhi et al. (2018) Injectable hyaluronic acid based microrods provide local micromechanical and biochemical cues to attenuate cardiac fibrosis after myocardial infarction. Biomaterials 169:11-21
Mkrtschjan, Michael A; Gaikwad, Snehal B; Kappenman, Kevin J et al. (2018) Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography. J Cell Physiol 233:3672-3683
Yan, Jiajie; Thomson, Justin K; Zhao, Weiwei et al. (2018) Role of Stress Kinase JNK in Binge Alcohol-Evoked Atrial Arrhythmia. J Am Coll Cardiol 71:1459-1470
Bohlooli Ghashghaee, Nazanin; Li, King-Lun; Solaro, R John et al. (2018) Role of the C-terminus mobile domain of cardiac troponin I in the regulation of thin filament activation in skinned papillary muscle strips. Arch Biochem Biophys 648:27-35
Yan, Jiajie; Zhao, Weiwei; Thomson, Justin K et al. (2018) Stress Signaling JNK2 Crosstalk With CaMKII Underlies Enhanced Atrial Arrhythmogenesis. Circ Res 122:821-835
Ait Mou, Younss; Lacampagne, Alain; Irving, Thomas et al. (2018) Altered myofilament structure and function in dogs with Duchenne muscular dystrophy cardiomyopathy. J Mol Cell Cardiol 114:345-353
Ferrantini, Cecilia; Coppini, Raffaele; Pioner, Josè Manuel et al. (2017) Pathogenesis of Hypertrophic Cardiomyopathy is Mutation Rather Than Disease Specific: A Comparison of the Cardiac Troponin T E163R and R92Q Mouse Models. J Am Heart Assoc 6:
Alves, Marco L; Warren, Chad M; Simon, Jillian N et al. (2017) Early sensitization of myofilaments to Ca2+ prevents genetically linked dilated cardiomyopathy in mice. Cardiovasc Res 113:915-925
Zak, Taylor J; Koshman, Yevgenia E; Samarel, Allen M et al. (2017) Regulation of Focal Adhesion Kinase through a Direct Interaction with an Endogenous Inhibitor. Biochemistry 56:4722-4731

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