The Gene Delivery Core will provide program investigators with complete in vitro and in vivo gene delivery solutions to facilitate completion of their specific aims. This Core is a critical component of our application as all projects will employ delivery of DNA constructs to endothelial cells and some will use recombinant protein delivery. The Core ensures availability of high quality custom molecular biology reagents such as adeno-, retro- and lentiviral vectors as well as various molecular biology services to investigators. The generation of these reagents is time-consuming and requires special skills and expertise. Having a Core facility in place that assures timely generation of custom molecular biology reagents allows investigators to focus efforts on their project's scientific direction. In the past funding cycle, the Gene Delivery Core has established a track record of developing new technologies tailored to emerging needs of the projects. In keeping with NIH data sharing plan, this Core also makes molecular reagents available to the scientific community at large at www.southalabama.edu/clb/genedelivery/genedelivery.htm.

Public Health Relevance

The Gene Delivery Core provides the molecular reagents to all projects that are necessary to complete the proposed studies. The Core is responsible for developing novel molecular approaches and reagents for transient and stable gene manipulation in pulmonary artery, capillary and vein endothelial cells, both in vitro and in vivo. These novel resources are made available to the general scientific community.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL066299-11A1
Application #
8293882
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2012-05-15
Budget End
2013-03-31
Support Year
11
Fiscal Year
2012
Total Cost
$306,392
Indirect Cost
$100,067
Name
University of South Alabama
Department
Type
DUNS #
172750234
City
Mobile
State
AL
Country
United States
Zip Code
36688
Leavesley, Silas J; Walters, Mikayla; Lopez, Carmen et al. (2016) Hyperspectral imaging fluorescence excitation scanning for colon cancer detection. J Biomed Opt 21:104003
Spadafora, Domenico; Kozhukhar, Natalia; Alexeyev, Mikhail F (2016) Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number. PLoS One 11:e0152705
Alvarez, Diego F; Housley, Nicole; Koloteva, Anna et al. (2016) Caspase-1 Activation Protects Lung Endothelial Barrier Function during Infection-Induced Stress. Am J Respir Cell Mol Biol 55:500-510
Morrow, K Adam; Ochoa, Cristhiaan D; Balczon, Ron et al. (2016) Pseudomonas aeruginosa exoenzymes U and Y induce a transmissible endothelial proteinopathy. Am J Physiol Lung Cell Mol Physiol 310:L337-53
Blair, Leslie A; Haven, April K; Bauer, Natalie N (2016) Circulating microparticles in severe pulmonary arterial hypertension increase intercellular adhesion molecule-1 expression selectively in pulmonary artery endothelium. Respir Res 17:133
Jian, Ming-Yuan; Liu, Yanping; Li, Qian et al. (2016) N-cadherin coordinates AMP kinase-mediated lung vascular repair. Am J Physiol Lung Cell Mol Physiol 310:L71-85
Shetewy, Aza; Shimada-Takaura, Kayoko; Warner, Danielle et al. (2016) Mitochondrial defects associated with β-alanine toxicity: relevance to hyper-beta-alaninemia. Mol Cell Biochem 416:11-22
Shokolenko, Inna N; Wilson, Glenn L; Alexeyev, Mikhail F (2016) The "fast" and the "slow" modes of mitochondrial DNA degradation. Mitochondrial DNA A DNA Mapp Seq Anal 27:490-8
Kozhukhar, Natalya; Spadafora, Domenico; Fayzulin, Rafik et al. (2016) The efficiency of the translesion synthesis across abasic sites by mitochondrial DNA polymerase is low in mitochondria of 3T3 cells. Mitochondrial DNA A DNA Mapp Seq Anal 27:4390-4396
Nguyen, Joanne M; Qualmann, Krista J; Okashah, Rebecca et al. (2015) 5p deletions: Current knowledge and future directions. Am J Med Genet C Semin Med Genet 169:224-38

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