The Gene Delivery Core will provide program investigators with complete in vitro and in vivo gene delivery solutions to facilitate completion of their specific aims. This Core is a critical component of our application as all projects will employ delivery of DNA constructs to endothelial cells and some will use recombinant protein delivery. The Core ensures availability of high quality custom molecular biology reagents such as adeno-, retro- and lentiviral vectors as well as various molecular biology services to investigators. The generation of these reagents is time-consuming and requires special skills and expertise. Having a Core facility in place that assures timely generation of custom molecular biology reagents allows investigators to focus efforts on their project's scientific direction. In the past funding cycle, the Gene Delivery Core has established a track record of developing new technologies tailored to emerging needs of the projects. In keeping with NIH data sharing plan, this Core also makes molecular reagents available to the scientific community at large at www.southalabama.edu/clb/genedelivery/genedelivery.htm.
The Gene Delivery Core provides the molecular reagents to all projects that are necessary to complete the proposed studies. The Core is responsible for developing novel molecular approaches and reagents for transient and stable gene manipulation in pulmonary artery, capillary and vein endothelial cells, both in vitro and in vivo. These novel resources are made available to the general scientific community.
|Sellak, Hassan; Zhou, Chun; Liu, Bainan et al. (2014) Transcriptional regulation of ?1H T-type calcium channel under hypoxia. Am J Physiol Cell Physiol 307:C648-56|
|Rich, Thomas C; Webb, Kristal J; Leavesley, Silas J (2014) Can we decipher the information content contained within cyclic nucleotide signals? J Gen Physiol 143:17-27|
|Favreau, Peter F; Hernandez, Clarissa; Heaster, Tiffany et al. (2014) Excitation-scanning hyperspectral imaging microscope. J Biomed Opt 19:046010|
|Villalta, Patricia C; Rocic, Petra; Townsley, Mary I (2014) Role of MMP2 and MMP9 in TRPV4-induced lung injury. Am J Physiol Lung Cell Mol Physiol 307:L652-9|
|Stevens, Trevor C; Ochoa, Cristhiaan D; Morrow, K Adam et al. (2014) The Pseudomonas aeruginosa exoenzyme Y impairs endothelial cell proliferation and vascular repair following lung injury. Am J Physiol Lung Cell Mol Physiol 306:L915-24|
|Favreau, Peter; Hernandez, Clarissa; Lindsey, Ashley Stringfellow et al. (2014) Thin-film tunable filters for hyperspectral fluorescence microscopy. J Biomed Opt 19:011017|
|Xu, Ningyong; Francis, Michael; Cioffi, Donna L et al. (2014) Studies on the resolution of subcellular free calcium concentrations: a technological advance. Focus on "detection of differentially regulated subsarcolemmal calcium signals activated by vasoactive agonists in rat pulmonary artery smooth muscle cells". Am J Physiol Cell Physiol 306:C636-8|
|Hashizume, Masahiro; Mouner, Marc; Chouteau, Joshua M et al. (2013) Mitochondrial-targeted DNA repair enzyme 8-oxoguanine DNA glycosylase 1 protects against ventilator-induced lung injury in intact mice. Am J Physiol Lung Cell Mol Physiol 304:L287-97|
|Alexeyev, Mikhail; Shokolenko, Inna; Wilson, Glenn et al. (2013) The maintenance of mitochondrial DNA integrity--critical analysis and update. Cold Spring Harb Perspect Biol 5:a012641|
|Balczon, Ron; Prasain, Nutan; Ochoa, Cristhiaan et al. (2013) Pseudomonas aeruginosa exotoxin Y-mediated tau hyperphosphorylation impairs microtubule assembly in pulmonary microvascular endothelial cells. PLoS One 8:e74343|
Showing the most recent 10 out of 73 publications