Core B is the Cell Culture and Small Animal Scientific Core whose goals are to provide Program Investigators with complete cell culture and cell isolation services (Cell Culture component) and a variety of small animal and microbiological services (Small Animal component) that facilitate the completion of their Specific Aims. To accomplish these goals, Core B will be centering its activities into three principal areas: Service component: Core B will facilitate the performance of each project by centralizing cell processing including isolation, characterization, and distribution of pulmonary endothelial cells (PAECs), microvascular endothelial cells (PMVECs), and pulmonary vein endothelial cells (PVECs) from wild type and genetically modified rats and mice, and by providing consultation and training in cell isolation and culture. Core B will facilitate the performance of each project by centralizing animal care including maintenance of mouse and rat colonies (breeding and colony expansion) of animals harboring genetic mutations, by maintaining bacterial stocks of Pseudomonas aeruginosa, and by generating P. aeruginosa-induced lung injury in rats and mice. Academic component: Throughout previous funding cycles, Core B has adapted to the ever-changing needs of the Projects by generating new reagents (e.g. providing pulmonary endothelial cells from genetically modified rats and mice) or platforms (e.g. providing an experimental rodent model of Pseudomonas aeruginosa-induced pneumonia and sepsis). During the current cycle, the Core has developed a novel experimental platform -Monolayer Stress Microscopy- to assess endothelial cell mechanics via visualization of forces occurring among cells and their subjacent substrate. In addition, the Core has generated mouse and rat decellularized lung bio-scaffolds that will serve as a platform for examining endothelial cell heterogeneity. Synergy with Projects and Scientific Cores: In collaboration with Core D (BioImaging and BioTechnology Implementation Core), pulmonary endothelial cells and isolated lung tissues will be provided for 3D, 4D, and 5D imaging analyses. Scientifically, the analysis of monolayer mechanical forces and the generation of lung scaffolds will become fundamental for data- and hypothesis- generation by providing innovative platforms to all Projects and Cores. In this capacity, scaffolds repopulated with genetically modified cells (provided from Core C) will be imaged using the novel approaches developed by Core D and the newly-acquired information will be disseminated in coordination with Core A (administrative Core).

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Xiao, Lei
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of South Alabama
United States
Zip Code
Shokolenko, Inna N; Alexeyev, Mikhail F (2017) Mitochondrial transcription in mammalian cells. Front Biosci (Landmark Ed) 22:835-853
Balczon, Ron; Morrow, K Adam; Zhou, Chun et al. (2017) Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J 31:2785-2796
Spadafora, Domenico; Kozhukhar, Natalia; Alexeyev, Mikhail F (2016) Presequence-Independent Mitochondrial Import of DNA Ligase Facilitates Establishment of Cell Lines with Reduced mtDNA Copy Number. PLoS One 11:e0152705
Jian, Ming-Yuan; Liu, Yanping; Li, Qian et al. (2016) N-cadherin coordinates AMP kinase-mediated lung vascular repair. Am J Physiol Lung Cell Mol Physiol 310:L71-85
Leavesley, Silas J; Walters, Mikayla; Lopez, Carmen et al. (2016) Hyperspectral imaging fluorescence excitation scanning for colon cancer detection. J Biomed Opt 21:104003
Shokolenko, Inna N; Wilson, Glenn L; Alexeyev, Mikhail F (2016) The ""fast"" and the ""slow"" modes of mitochondrial DNA degradation. Mitochondrial DNA A DNA Mapp Seq Anal 27:490-8
Kozhukhar, Natalya; Spadafora, Domenico; Fayzulin, Rafik et al. (2016) The efficiency of the translesion synthesis across abasic sites by mitochondrial DNA polymerase is low in mitochondria of 3T3 cells. Mitochondrial DNA A DNA Mapp Seq Anal 27:4390-4396
Francis, Michael; Xu, Ningyong; Zhou, Chun et al. (2016) Transient Receptor Potential Channel 4 Encodes a Vascular Permeability Defect and High-Frequency Ca(2+) Transients in Severe Pulmonary Arterial Hypertension. Am J Pathol 186:1701-9
Alvarez, Diego F; Housley, Nicole; Koloteva, Anna et al. (2016) Caspase-1 Activation Protects Lung Endothelial Barrier Function during Infection-Induced Stress. Am J Respir Cell Mol Biol 55:500-510
Morrow, K Adam; Ochoa, Cristhiaan D; Balczon, Ron et al. (2016) Pseudomonas aeruginosa exoenzymes U and Y induce a transmissible endothelial proteinopathy. Am J Physiol Lung Cell Mol Physiol 310:L337-53

Showing the most recent 10 out of 115 publications