Core A, Microscopy, comprises valuable, electron and modern light microscopic tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. Core A was established in the original application to meet the growing needs of the investigators in this area and has expanded in this renewal to include Dr. Farquhar as the core leader bringing her expertise as a microscopist due to the recent retirement of Dr. Feramisco. Electron microscopy facilities under her direction are located in the Center for Molecular Medicine and contain state of the art microscopes and specialized preparatory equipment. The core also includes Dr. Patel as a co-investigator who has expertise in light microscopy and has worked over ten years with Dr. Feramisco and the newly established School of Medicine (SOM) Microscopy Core which includes 2- photon confocal, spinning disk, super-resolution (purchase planned) and deconvolution systems. We propose in this application to fund two part-time research assistants to provide dedicated support to PPG members by 1) providing standardized training in the use of electron microscopy and 3-D fluorescence microscopes, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of the many microscopes available. With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed Core A will facilitate the ability of PPG members to access these services in a timely and cost-efficient manner.
Core A will provide basic and sophisticated tools including multiple microscopes, important training and, experimental design for PPG members. The goal will be to provide the best possible resources to visualize proteins and protein-protein interactions inside a cell specific to each project and experimental situation in a timely and cost effective manner.
|Pfeiffer, E R; Wright, A T; Edwards, A G et al. (2014) Caveolae in ventricular myocytes are required for stretch-dependent conduction slowing. J Mol Cell Cardiol 76:265-74|
|Wang, Jiawan; Schilling, Jan M; Niesman, Ingrid R et al. (2014) Cardioprotective trafficking of caveolin to mitochondria is Gi-protein dependent. Anesthesiology 121:538-48|
|Lee, Kristen L; Hoey, David A; Spasic, Milos et al. (2014) Adenylyl cyclase 6 mediates loading-induced bone adaptation in vivo. FASEB J 28:1157-65|
|Tang, Tong; Lai, N Chin; Wright, Adam T et al. (2013) Adenylyl cyclase 6 deletion increases mortality during sustained *-adrenergic receptor stimulation. J Mol Cell Cardiol 60:60-7|
|Gao, Mei Hua; Lai, Ngai Chin; Tang, Tong et al. (2013) Preserved cardiac function despite marked impairment of cAMP generation. PLoS One 8:e72151|
|Tang, Tong; Hammond, H Kirk (2013) Gene transfer for congestive heart failure: update 2013. Transl Res 161:313-20|
|Rieg, Timo; Tang, Tong; Uchida, Shinichi et al. (2013) Adenylyl cyclase 6 enhances NKCC2 expression and mediates vasopressin-induced phosphorylation of NKCC2 and NCC. Am J Pathol 182:96-106|
|Gao, Mei Hua; Lai, N Chin; Miyanohara, Atsushi et al. (2013) Intravenous adeno-associated virus serotype 8 encoding urocortin-2 provides sustained augmentation of left ventricular function in mice. Hum Gene Ther 24:777-85|
|Lai, N Chin; Tang, Tong; Gao, Mei Hua et al. (2012) Improved function of the failing rat heart by regulated expression of insulin-like growth factor I via intramuscular gene transfer. Hum Gene Ther 23:255-61|
|Tang, T; Gao, M H; Hammond, H Kirk (2012) Prospects for gene transfer for clinical heart failure. Gene Ther 19:606-12|
Showing the most recent 10 out of 54 publications