The Translational Systems Core (Core C) will interface with the Project Leaders (Hammond, Roth, and Dillmann), Core A (Farquhar) and Core B (Miyanohara). This shared resource is designed to provide Program scientists, in a single facility, specific techniques that are labor intensive, highly specialized, not generally available to individual laboratories, and expensive. The specific translational systems provided by this Core include detailed cardiovascular physiological studies, the development of cardiovascular disease models, virus vector gene transfer methods, and detailed analysis of excitation-contraction coupling. Physiological assessment will include: i) Transthoracic echocardiography to evaluate cardiac chamber size, wall thickness, and function in vivo;2) Assessment of contractile function of the heart, including measurement of the end-systolic pressure-volume relationship;3) The study of isolated perfused hearts, which will allow more refined and specific measurements of LV contractility in a controlled setting isolated from complex reflex activation and adrenergic activation associated with surgical preparations;4) Telemetry to assess blood pressure and ECG in ambulatory rodents. 4) Detailed cardiac myocyte calcium handling analysis, performed via patch-clamp methods, will be performed at UCLA by a collaborator, Dr Joshua Goldhaber. In addition to providing these studies for all Program Scientists, the Translational Systems Core will provide a highly cost efficient means to conduct such studies in a centralized and well-equipped location, circumventing the need for redundant efforts of individual participating laboratories. By consolidating physiological model development and assessment and viral gene transfer, the cost savings will be considerable.

Public Health Relevance

Translational Core is constantly evolving to bring in state of the art technologies, devise new instrumentation procedures, and develop new disease models in keeping pace with new advances in science and technology.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL066941-12
Application #
8743239
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
12
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Veterans Medical Research Fdn/San Diego
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92161
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Hammond, H Kirk; Penny, William F; Traverse, Jay H et al. (2016) Intracoronary Gene Transfer of Adenylyl Cyclase 6 in Patients With Heart Failure: A Randomized Clinical Trial. JAMA Cardiol 1:163-71
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