Abstract

SC2 Scientific Core 2 expresses proteins that are essential for the three Projects of the PPG. The proteins are expressed primarily in bacteria and also insect cells. The proteins are then purified and characterized to test that they are properly folded and functionally active. SC2 also constructs vectors for expression of proteins in mammalian cells. Sequences of all constructs are verified. These high-quality reagents are then turned over to the projects for utilization in proposed experiments. Over 50 proteins and 50 DNA vectors will be constructed in the Core. By centralizing these activities, common reagents and expertise can be shared and consistent behavior of shared reagents and outcomes can be assured. Scientific Core 2 also provides training to PPG personnel in to protein expression in bacteria and mammalian cells, protein purification and characterization and in mutagenesis strategies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL073311-15
Application #
9676305
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Sarkar, Rita
Project Start
Project End
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
15
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195

  Publications

Szpak, Dorota; Izem, Lahoucine; Verbovetskiy, Dmitriy et al. (2018) ?M?2 Is Antiatherogenic in Female but Not Male Mice. J Immunol 200:2426-2438
Plow, Edward F; Wang, Yunmei; Simon, Daniel I (2018) The search for new antithrombotic mechanisms and therapies that may spare hemostasis. Blood 131:1899-1902
Sossey-Alaoui, Khalid; Pluskota, Elzbieta; Szpak, Dorota et al. (2018) The Kindlin-2 regulation of epithelial-to-mesenchymal transition in breast cancer metastasis is mediated through miR-200b. Sci Rep 8:7360
Gao, Detao; Podrez, Eugene A (2018) Characterization of covalent modifications of HDL apoproteins by endogenous oxidized phospholipids. Free Radic Biol Med 115:57-67
Biswas, Sudipta; Zimman, Alejandro; Gao, Detao et al. (2017) TLR2 Plays a Key Role in Platelet Hyperreactivity and Accelerated Thrombosis Associated With Hyperlipidemia. Circ Res 121:951-962
Wang, Yunmei; Gao, Huiyun; Shi, Can et al. (2017) Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIb?. Nat Commun 8:15559
Meller, Julia; Chen, Zhihong; Dudiki, Tejasvi et al. (2017) Integrin-Kindlin3 requirements for microglial motility in vivo are distinct from those for macrophages. JCI Insight 2:
Hirbawi, Jamila; Bialkowska, Katarzyna; Bledzka, Kamila M et al. (2017) The extreme C-terminal region of kindlin-2 is critical to its regulation of integrin activation. J Biol Chem 292:14258-14269
Ithychanda, Sujay S; Dou, Kevin; Robertson, Stephen P et al. (2017) Structural and thermodynamic basis of a frontometaphyseal dysplasia mutation in filamin A. J Biol Chem 292:8390-8400
Feng, Weiyi; Valiyaveettil, Manojkumar; Dudiki, Tejasvi et al. (2017) ?3 phosphorylation of platelet ?IIb?3 is crucial for stability of arterial thrombus and microparticle formation in vivo. Thromb J 15:22

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