The objective of the Animal/Histomorphometry core is to provide investigators of the program project grant (PPG) scientific, technical and financial support for the design and execution of studies involving animals and histomorphometric assessment of cardiac tissue. Our state-of-the art facility houses and contains all the animals and equipment necessary to execute experiments and analyze specimens from dogs with coronary microembolization-induced heart failure (HF) as well as from dogs with rapid pacing-induced HF. Over the past 23 years, we have developed and fully characterized a canine model of chronic HF which is ideally suited for the studies outlined in the PPG. Heart failure in this model is produced by multiple sequential intracoronary embolizations with microspheres that lead to loss of viable myocardium. The model manifests many, if not all of the sequelae of HF in humans including profound systolic and diastolic LV dysfunction, LV dilation and compensatory hypertrophy, increased LV filling pressures, increased systemic vascular resistance, decreased cardiac output and, importantly, abnormalities of mitochondria and characteristic progressive worsening of LV function. The Core will the perform the following aims: 1) coordinate the acquisition, housing and preparation of dogs involved in the coronary microembolization-induced HF studies, 2) execute pharmacologic protocols involving coronary microembolization-induced HF i.e. assume responsibility for treatment regimens for project 2, 3) coordinate distribution of dogs and cardiac tissues derived from coronary microembolization-induced HF experiments and 4) perform histological analysis of tissue samples derived from coronary-microembolization induced HF and mouse studies (Projects 1, 2 and 4) as well as from dogs with rapid-pacing-induced HF that are specific to project 3 of the PPG. Services to PPG investigators will be provided by a team consisting of the Core Leader (Dr. H.N. Sabbah), the Core Co- Leader (Dr. V.G. Sharov), 1 Assistant Staff Investigator (Dr M. Wang) and 1 Licensed Veterinary Technologists (Ms. Annette Hazel). The facility veterinarian (Dr. Gregory Heisey) is an institutional resource and will provide all the necessary and required veterinary support without charge.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Henry Ford Health System
United States
Zip Code
Flori, Alessandra; Liserani, Matteo; Frijia, Francesca et al. (2015) Real-time cardiac metabolism assessed with hyperpolarized [1-(13) C]acetate in a large-animal model. Contrast Media Mol Imaging 10:194-202
Vimercati, Claudio; Qanud, Khaled; Mitacchione, Gianfranco et al. (2014) Beneficial effects of acute inhibition of the oxidative pentose phosphate pathway in the failing heart. Am J Physiol Heart Circ Physiol 306:H709-17
Mitacchione, Gianfranco; Powers, Jeffrey C; Grifoni, Gino et al. (2014) The gut hormone ghrelin partially reverses energy substrate metabolic alterations in the failing heart. Circ Heart Fail 7:643-51
Velez, Mauricio; Kohli, Smita; Sabbah, Hani N (2014) Animal models of insulin resistance and heart failure. Heart Fail Rev 19:1-13
Prosdocimo, Domenick A; Anand, Priti; Liao, Xudong et al. (2014) Kruppel-like factor 15 is a critical regulator of cardiac lipid metabolism. J Biol Chem 289:5914-24
Shekar, Kadambari Chandra; Li, Ling; Dabkowski, Erinne R et al. (2014) Cardiac mitochondrial proteome dynamics with heavy water reveals stable rate of mitochondrial protein synthesis in heart failure despite decline in mitochondrial oxidative capacity. J Mol Cell Cardiol 75:88-97
Clericò, Vito; Masini, Luca; Boni, Adriano et al. (2014) Water-dispersible three-dimensional LC-nanoresonators. PLoS One 9:e105474
Rosca, Mariana G; Tandler, Bernard; Hoppel, Charles L (2013) Mitochondria in cardiac hypertrophy and heart failure. J Mol Cell Cardiol 55:31-41
Hecker, Peter A; Lionetti, Vincenzo; Ribeiro Jr, Rogerio F et al. (2013) Glucose 6-phosphate dehydrogenase deficiency increases redox stress and moderately accelerates the development of heart failure. Circ Heart Fail 6:118-26
Galvao, Tatiana F; Khairallah, Ramzi J; Dabkowski, Erinne R et al. (2013) Marine n3 polyunsaturated fatty acids enhance resistance to mitochondrial permeability transition in heart failure but do not improve survival. Am J Physiol Heart Circ Physiol 304:H12-21

Showing the most recent 10 out of 139 publications